We have studied the effects of murine cytomegalovirus (MCMV) infection on bone marrow stem and progenitor cell populations to find an explanation for the defects in hematopoiesis that accompany CMV infections in patients. Sublethal MCMV infection of BALB/c mice resulted in a 5- to 10-fold decrease in the numbers of myeloid (colony-forming unit-granulocyte-macrophage [CFU-GM]) and erythroid (bur...

Human erythroblastic progenitors (colony-forming unit-erythroid [CFU-E] and burst-forming unit-erythroid [BFU-E]) have been shown to attach to fibronectin (Fn), a property that might be involved in the local regulation of erythropoiesis. In this study, we have investigated changes in cell attachment to Fn upon terminal erythroid differentiation. We first purified CFU-E from human marrow by avid...

The extent to which mouse megakaryoseparate megakaryocyte progenitor cells cyte progenitor cells (colony-forming unitmay be biased in their capacity for promegakaryocyte, CFU-M) can proliferate in liferation versus endomitosis. Differences semisolid cultures prior to endomitosis, were observed in the cell cycle characand conditions that may regulate that difteristics of CFU-M as determined in v...

Antibody L4F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM, CFU-Meg, BFU-E, and CFU-Mix. In the present study we examined whether cells that do not express the L4F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with L4F3 and com...

Antibody 14F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM. CFU-Meg, BFU-E, and CFUMix. In the present study we examined whether cells that do not express the 14F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with l4F3 and comp...

The extent to which mouse megakaryoseparate megakaryocyte progenitor cells cyte progenitor cells (colony-forming unitmay be biased in their capacity for promegakaryocyte, CFU-M) can proliferate in liferation versus endomitosis. Differences semisolid cultures prior to endomitosis, were observed in the cell cycle characand conditions that may regulate that difteristics of CFU-M as determined in v...

OBJECTIVES Bisphosphonates (BPN) have actions on a variety of cell types including: osteoclasts, osteoblasts, osteocytes, and endothelial cells. The objectives of this report are to review the current state of understanding of the effects of BPNs on orthodontic tooth movement and to provide evidence on BPN's in vivo effects on bone marrow-derived osteoprogenitor cells. MATERIAL AND METHODS Mi...

We investigated the in vitro hematopoietic stimulatory activity of leukemia inhibitory factor/human interleukin for DA cells (LIF/HILDA) on bone marrow progenitor populations in 17 normal individuals. In serum-free cultures LIF/HILDA did not induce colony growth. In serum containing media, LIF/HILDA stimulated the growth of colony forming unit (CFU)-MIX and CFU-EO in a dose-dependent fashion an...