Background & Aims of the Study: Leachate production is a major problem for solid waste landfills and causes important threat to health public and environment. One of the typical properties of heavy metals is that they cannot easily decompose in the environment. The aim of this study was to measure of heavy metals concentrations in old-age leachate from Qazvin landfill site, Ira...

Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic feature...

UNLABELLED The molecular integrity of the active site of phytases from fungi is critical for maintaining phytase function as efficient catalytic machines. In this study, the molecular dynamics (MD) of two monomers of phytase B from Aspergillus niger, the disulfide intact monomer (NAP) and a monomer with broken disulfide bonds (RAP), were simulated to explore the conformational basis of the loss...

α-amylase has been studied extensively from various sides. this enzyme is used in many industries .many applications of this enzyme have encouraged us for greater attempts on the study of α-amylase and to search for more effective processes. in this investigation, the structure of nanotube - catalytic site of bacillus subtilis α- amylase was optimized by hyperchem 7.0 and then it was investigat...

Description cPLA2alpha is an 85 kDa protein consisting of 749 amino acids. cPLA2alpha belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). The protein contains an N-terminal C2 domain (Cadependent, phospholipid binding) and two catalytic domains (al...

The crystal structure of the esterase EstA from the cold-adapted bacterium Pseudoalteromonas sp. 643A was determined in a covalently inhibited form at a resolution of 1.35 A. The enzyme has a typical SGNH hydrolase structure consisting of a single domain containing a five-stranded beta-sheet, with three helices at the convex side and two helices at the concave side of the sheet, and is ornament...

The locations of the catalytic and receptor-binding domains of the Pasteurella multocida toxin (PMT) were investigated. N- and C-terminal fragments of PMT were cloned and expressed as fusion proteins with affinity tags. Purified fusion proteins were assessed in suitable assays for catalytic activity and cell-binding ability. A C-terminal fragment (amino acids 681 to 1285) was catalytically acti...

background & aims of the study: leachate production is a major problem for solid waste landfills and causes important threat to health public and environment. one of the typical properties of heavy metals is that they cannot easily decompose in the environment. the aim of this study was to measure of heavy metals concentrations in old-age leachate from qazvin landfill site, iran. materials & me...

The accessory Sec system is a specialized transport system that exports serine-rich repeat (SRR) glycoproteins of Gram-positive bacteria. This system contains two homologues of the general secretory (Sec) pathway (SecA2 and SecY2) and several other essential proteins (Asp1 to Asp5) that share no homology to proteins of known function. In Streptococcus gordonii, Asp2 is required for the transpor...