Erythrocytes obtained from patients who manifest autoimmune hemolytic anemia can be divided into at least three categories by the nature of their protein coats as determined by direct antiglobulin (Coombs) test: IgG alone, IgG and complement (C), C alone. IgG antibodies were detected by direct Coombs Test at 4#{176}Cbut not at 37#{176}Cin patients of type 3 A.H.A. Experiments at 4, 10, 15, 20, ...

Background: Brucellosis is one of the important zoonotic diseases with variety of symptoms and signs. Using of cost effective, easy and safe diagnostic methods in screening of brucellosis is an important priority. Several studies have been carried out to recognize appropriate diagnostic methods and each have reported different results. Aim: The aim of this study was to investigate the sensitivi...

Chronic hepatosplenic suppurative brucellosis (CHSB) is a local reactivation of a previous brucellosis, coursing with an immunoglobulin G (IgG) and IgA secondary immunological response. The observation of two cases of CHSB with an apparent IgM response gave rise to a detailed serological study of three of our patients. We studied the first sample from all three patients and successive samples f...

BACKGROUND Association of autoimmune haemolytic anaemia has been seldom reported with Kawasaki disease. CASE CHARACTERISTICS A 7-month-old boy, presented with prolonged fever, erythematous rash, severe pallor and hepatosplenomegaly. OBSERVATIONS Positive Direct Coombs test and coronary artery aneurysm on echocardiography. He was managed with steroids along with intravenous immunoglobulins a...

The calculating method for fuel consumption (FC) was put forward and calibrated and the characteristics of the fuel consumption on intersections were analyzed based on 18 groups of vehicular operating data which were acquired from the test experiment of a single vehicle along the urban arterial roads in Harbin, China. The results obtained show that 50.36% of the fuel consumption for the test ve...

THORNLEY, Wilson, Hormaeche, Oates and Coombs (1979) used a method of solid-phase radioimmunoassay (RIA) devised for brucella antigens (Wilson, Thornley and Coombs, 1977) to detect gonococcal antigens in a buffer system. When this test was used to detect gonococcal antigens in urine samples, many were found to be strongly inhibitory. This paper describes a study of some of the properties of the...

It was shown by Wiener (1944) that some human a,nti-Rh sera contained incomplete antibodies which combined with but did not agglutinate the corresponding red cells. They appeared to be more avid than agglutinating antibodies, so that, if the two kinds of antibody co-existed in the same serum, the incomplete or blocking antibody might account for failure of agglutination or for a zone of inhibit...

Autoimmune hemolytic anemia is characterized by shortened red blood cell survival and a positive Coombs test. The responsible autoantibodies may be either warm reactive or cold reactive. The rate of hemolysis and the severity of the anemia may vary from mild to severe and life-threatening. Diagnosis is made in the laboratory by the findings of anemia, reticulocytosis, a positive Coombs test, an...

The main objectives of this study were to determine the occurrence and potential causative factors of Immune-mediated hemolytic anemia (IMHA) in native cattle and water buffaloes from southwest of Iran. Fifty-three anemic animals (37 cattle and 16 buffaloes) were studied. A full clinical history and physical examinations were undertaken for all animals. Four clinically healthy cattle and four h...