many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. the proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. using primary fetal liver cells, we established...

background: in this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. materials and methods: human umbilical cord blood-derived mesenchymal stem cells were first cultured in dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incub...

new blood forming vessels are produced by differentiation of mesodermal precursor cells to angioblasts that become endothelial cells (ecs) which in turn give rise to primitive capillary network. human cord blood (hcb) contains large subsets of mononuclear cells (mncs) that can be differentiated into endothelial-like cells in vitro. human mononuclear progenitor cells were purified from fresh umb...

Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...

abstract background b cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. it has been shown that b cells produced successfully in the presence of stem cell factor (scf) and flt3 ligand (flt3l). il2, il7, and il15 cytokines as  -common family have an essential cytokines may support either synergistically or in an additi...

Introduction: Human hematopoietic stem cells (hHSCs) have been used for transplantation in hematologic failures. Because the number of hHSCs per cord blood unit is limited, the expansion of these cells is important for clinical application. It has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hHSCs. In this regard, cord blood CD34+ cells ex...

New blood forming vessels are produced by differentiation of mesodermal precursor cells to angioblasts that become endothelial cells (ECs) which in turn give rise to primitive capillary network. Human cord blood (HCB) contains large subsets of mononuclear cells (MNCs) that can be differentiated into endothelial-like cells in vitro. Human mononuclear progenitor cells were purified from fresh umb...

introduction: human hematopoietic stem cells (hhscs) have been used for transplantation in hematologic failures. because the number of hhscs per cord blood unit is limited, the expansion of these cells is important for clinical application. it has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hhscs. in this regard, cord blood cd34+ cells expanded a...

Cord blood is a sample of blood taken from a newborn baby's umbilical cord.Cord blood is collected because it is rich in stem cells content, such as hematopoietic cells, which can be used to treat hematopoietic and genetic disorders. Cord Blood Transplantation has been used in the treatment of over 80 diseases, including leukemia, lymphoma and anemia. Advantages of Cord Blood Treatment: • ...