PURPOSE The purpose of this study was to assess the ability of quantitative in vivo confocal microscopy (CM) to detect changes in cystine crystal volume in the cystinosisn (Ctns(-/-))mouse cornea following topical cysteamine therapy. METHODS Fifteen Ctns(-/-) mice were sequentially followed using in vivo CM from 3 to 10 months of age. In a second experiment, five mice receiving topical cystea...

baradaran rafii ar, md; motevasseli t, md*; yazdizadeh f, md; ansari sh, md; karimian f, md; sadoughi mm, md; kiani h ocular tissue engineering research center, shahid beheshti university of medical sciences, tehran, iran * corresponding author: [email protected] purpose: to compare anterior segment indices measured by two scheimpflug camera; galilei and pentacam. methods: in this observa...

PURPOSE To investigate the therapeutic effects of mineral oil (MO) and hyaluronic acid (HA) mixture eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE). METHODS Eye drops consisting of 0.1% HA alone or mixed with 0.1%, 0.5%, or 5.0% MO were applied to desiccating stress-induced murine dry eyes. Tear volume, corneal irregularity score, tear film break-u...

Corneal blindness is considered to be a serious public health problem worldwide. It is estimated that 1.2–2 million cases of corneal blindness occur globally in a year and 90% of them are from developing countries (Chirambo et al., 1986; Whitcher et al., 1997). A survey by the Government of India estimates that corneal lesions are responsible for significant blindness cases (National Survery on...

Corneal diseases constitute the second leading cause of vision loss and affect more than 10 million people globally. As there is a severe shortage of fresh donated corneas and an unknown risk of immune rejection with traditional heterografts, it is very important and urgent to construct a corneal equivalent to replace pathologic corneal tissue. Corneal tissue engineering has emerged as a practi...

BACKGROUND High volume cataract surgery is practised in many eye centres. OBJECTIVE To investigate whether routine high volume cataract surgery can be performed without compromising the quality of surgery. MATERIALS AND METHODS A retrospective interventional study was carried out at a high volume eye care centre including 368 subjects with cataract operated within 5 randomly selected theatr...

PURPOSE To characterize the osmoprotective properties of L-carnitine on human corneal epithelial cell volume and apoptosis during hyperosmotic stress. METHODS Human corneal limbal epithelial (HCLE) cells were exposed to culture medium at 300 mOsm (isotonic) or 500 mOsm (hyperosmotic) with or without L-carnitine (10 mM). Induction of apoptosis was detected by quantifying the proteolytic activi...