Flow cytometry (FCM) is a rapid and sensitive technique that can determine cell numbers and measure various physiological characteristics of individual cells by using appropriate fluorescent probes. Previously, we developed an FCM assay with the viability probes carboxyfluorescein diacetate (cFDA) and TOTO-1 [1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methyl-2,3dihydro(benzo-1,3...

Twenty-five (10 ruminally cannulated) Holstein cows averaging 82 +/- 34 d in milk were assigned to 5 x 5 Latin squares (21-d periods) and fed diets supplemented with one of four different proteins to assess effects on production, ruminal metabolism, omasal flow of N fractions, and degradation rates of protein supplements. Total mixed diets contained (dry matter basis) 44% corn silage, 22% alfal...

Two studies were conducted to evaluate the effect of a glyphosate-tolerant (event nk603) and a corn rootworm protected (event MON863) corn hybrid on feed intake and milk production compared with the nontransgenic hybrid and two reference hybrids. In Experiment 1, 16 multiparous Holstein cows were assigned to one of four treatments in replicated 4 x 4 Latin squares with 28-d periods. Diets conta...

Total mixed rations containing 31 or 25% NDF were supplemented with 0 or .5 kg/cow per d Ca salts of fatty acids to study the effect of adding Ca salts of fatty acids to diets that differed in NDF content. Rations were fed for ad libitum intake to 12 early to midlactation Holstein cows in a replicated 4 x 4 Latin square design with a 2 x 2 factorial arrangement of treatments. No significant int...

some factors affecting crude protein balance (cpb), as a measure of efficiency, were studied using 2824 records of 501 lactating holstein cows. the cpb (kg d-1) was calculated as crude protein (cp) intake (kg) minus cp yield (kg). two different statistical methods including principal components (pcs) and general linear model analyses were used to study the effects of different factors. crude pr...

A study was conducted using a randomized complete block design to determine feed intake, live-weight changes, milk yield and cost of milk production in Friesian cows fed napier grass (NG) at 10 weeks (MNG) or 15 weeks (ONG) of maturity. The MNG or ONG was supplemented with equal amounts of sunflower (SFBC) or poultry litter (PLBC) based concentrates in experiment 1, while in experiment 2, the M...

We previously reported that milk production by dairy cows was increased by adding specific xylanase-rich (XYL) and xylanase-cellulase enzymes to corn silage-based diets containing 0 or 10% bermudagrass silage. This study examined effects of adding XYL on the intake and performance of lactating dairy cows consuming a totally mixed ration (TMR) formulated with a greater proportion of bermudagrass...

The dairy cows experience negative energy balance during early lactation and feeding Bypass fat in ration can produce higher milk yield, improved reproduction with better economics. An experiment was planned to evaluate of total mixed (TMR) supplemented @ 100 g/day animals for -21 120 days calving. Total sixteen were divided control group having eight each. effect on production, economics evalu...

1 Dept of Medicine Animal, Production and Health Padova Univ., Agripolis, Viale dell’Università, 16, 35020 Legnaro (PD), Italy 2 Istituto Zooprofilattico delle Venezie, Viale dell’Università, 10, 35020 Legnaro (PD), Italy ABSTRACT The study aimed at evaluating the effect of a daily dose of 14 g of choline administrated by using soy lecithins (LC group), a by-product of the biodiesel production ...

The objectives of this study were to measure particle size and evaluate the effect of increasing alfalfa hay particle size on production characteristics in lactating Holstein dairy cows. Ninety multiparous Holstein cows in early to mid-lactation were randomly assigned in a complete randomized design for a 30-day period. Animals were offered one of the three diets, which were identical in energy...