p0005 Corticotropin-releasing hormone [CRH; also referred to as corticotropin-releasing factor (CRF)] is a member of a family of related peptides in vertebrates that includes the fish urotensins-I, frog sauvagine, and the urocortin/stresscopin peptides. CRF was first named for its stimulatory effect on corticotropin [also known as adrenocorticotropic hormone (ACTH)] secretion from the anterior ...

Using reversed-phase "high-performance" liquid chromatography and hydrophilic ion-pairing reagents, we studied the chromatographic profiles of corticotropin immunoreactivities in human plasma, cerebrospinal fluid, and urine. The chromatographic system we applied provides excellent separation of peptide molecules, with reproducibility (CV) of retention times better than 0.5%, and makes feasible ...

The clinical evidence for the harmful effect of cortisone on patients infected with tubercle bacilli (American Trudeau Society, 1952; Fred et al., 1951; Traut and Ellman, 1952) has been supported by a series of laboratory studies on mice (Hart and Ress, 1950), rats (Cummings et al., 1952; Michael et al., 1950), guinea pigs (Spain and Molomut, 1950), and rabbits (Lurie et al., 1951). In the prec...

Background: Adrenal insufficiency is frequently neglected and underappreciated, potentially severe complication of systemic glucocorticoid therapy. Objectives: We aimed to evaluate the prevalence induced adrenal in giant cell arteritis (GCA). Methods: analysed function data a cohort GCA patients diagnosed between July 2014 2019, whom discontinuation methylprednisolone therapy was planned. teste...

This study deals with the interaction of polypeptide hormones with their receptors. Specifically it involves the binding of synthetic ACTH analogs and fragments to a particulate fraction from beef adrenal cortical tissue. This fraction was found to bind synthetic [(14)C-Phe] [Gln(5)]beta-corticotropin(1-20) amide but failed to bind significant amounts of [(14)C-Phe] [Gln(5)]beta-corticotropin(1...

Corticotropin releasing factor and vasopressin were measured in major brain regions including the neurohypophysis in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) during development of hypertension. The highest concentration of corticotropin releasing factor was found in the hypothalamus in both strains. Corticotropin releasing factor was decreased in most major...

Using a specific and sensitive radioimmunoassay involving extraction with diethyl ether and chromatographic separation of steroids, we measured concentrations of salivary and plasma dehydroepiandrosterone (DHEA) in 22 women with normal ovulatory cycles (ages 18-45 years). Salivary DHEA values closely correlated with total and free DHEA in plasma. In the follicular phase the mean concentrations ...

A 2.5-fold stimulation of cyclic AMP cellular content is measured 60 sec after addition of 100 nM synthetic ovine corticotropin-releasing factor (C-RF; corticoliberin) to rat anterior pituitary cells in culture. A maximal response of cyclic AMP content at 400% above control is observed between 2 and 30 min after addition of the peptide, whereas an 8-fold stimulation of cyclic AMP released into ...

The aim of this study, comprising 88 suicide attempters, was to identify hypothalamic-pituitary-adrenal (HPA) -axis coupled CpG-sites showing methylation shifts linked to severity of the suicide attempt. Candidate methylation loci were further investigated as risk loci for a general psychiatric risk score in two cohorts of adolescents (cohort 1 and 2). The genome-wide methylation pattern was me...