نتایج جستجو برای: count normalization
تعداد نتایج: 176138 فیلتر نتایج به سال:
MicroRNAs (miRNAs) are short, endogenous non-coding RNAs that function as guide molecules to regulate transcription of their target messenger RNAs. Several methods including low-density qPCR arrays are being increasingly used to profile the expression of these molecules in a variety of different biological conditions. Reliable analysis of expression profiles demands removal of technical variati...
BACKGROUND Successful combination antiretroviral therapy (cART) increases levels of CD4+ T-cells, however this increase may not accurately reflect long-term immune recovery since T-cell dysregulation and loss of T-cell homeostasis often persist. We therefore assessed the impact of a decade of effective cART on immune regulation, T-cell homeostasis, and overall T-cell phenotype. METHODS We con...
Various biases affect high-throughput sequencing read counts. Contrary to the general assumption, we show that bias does not always cancel out when fold changes are computed and that bias affects more than 20% of genes that are called differentially regulated in RNA-seq experiments with drastic effects on subsequent biological interpretation. Here, we propose a novel approach to estimate fold c...
Sequencing of transposon-mutant libraries using next-generation sequencing (Tn-Seq) has become a popular method for determining which genes and non-coding regions are essential for growth under various conditions in bacteria. For methods that rely on comparison of read-counts at transposon insertion sites, proper normalization of Tn-Seq datasets is vitally important. Real Tn-Seq datasets often ...
IMPORTANCE In individuals with human immunodeficiency virus 1 (HIV-1) infection who are receiving antiretroviral therapy (ART), factors that promote full immune recovery are not well characterized. OBJECTIVE To investigate the influence of the timing of ART relative to HIV-1 infection on normalization of CD4+ T-cell counts, AIDS risk, and immune function. DESIGN, SETTING, AND PARTICIPANTS P...
Thrombopoietin (TPO) deficiency has been proposed as an important etiologic factor for thrombocytopenia in advanced-stage liver disease. To clarify the contributions of platelet production, platelet consumption, coagulation activation, and splenic sequestration to thrombocytopenia in liver disease, we studied TPO serum levels and markers of platelet production, platelet activation, and coagulat...
تغییرات غلظت گاز رادون درون آبهای زیرزمینی، یکی از پیش نشانگرهای شناخته شده زلزله می باشد. در گذشته روش های متعددی جهت اندازه گیری غلظت رادون درون آب ارائه شده است. در این پایان نامه با استفاده از کد mcnpx امکان اندازه گیری مستقیم تغییرات گاز رادون درون آب با استفاده از آشکارساز گایگر مولر مورد بررسی قرار گرفته است. برای این منظور یک آشکارساز گایگر مولر قرار گرفته در استوانه ای تفلونی که در آب ...
Standard expression microarray analysis consists of a number of different steps to enable signal comparisons between experimental conditions. The first step entails normalizing the signal intensity distributions of all probe features on all arrays. A probe feature is a location on the array that contains many copies of the same 25-mer DNA sequence. Normalizing these distributions enables compar...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید