Clear-sky brightness temperatures (BT) in five bands of the Advanced Himawari Imager (AHI; flown onboard Himawari-8 satellite) centered at 3.9, 8.6, 10.4, 11.2, and 12.3 μm (denoted by IR37, IR86, IR10, IR11, and IR12, respectively) are used in the NOAA Advanced Clear-Sky Processor for Oceans (ACSPO) sea surface temperature (SST) retrieval system. Here, AHI BTs are preliminarily evaluated for s...

Staphyloxanthin (STX), a golden carotenoid pigment produced by Staphylococcus aureus, is suggested to act as an important virulence factor due to its antioxidant properties. Restraining biosynthesis of STX was considered as an indicator of virulence decline in pigmented S. aureus isolates. However, it is not clear whether natural nonpigmented S. aureus isolates have less virulence than pigmente...

The accuracy of brightness temperature (BT) from the Cross-track Infrared Sounder (CrIS) onboard Suomi National Polar-orbiting Partnership (S-NPP) satellite and NOAA-20 is estimated using Constellation Observing System for Meteorology, Ionosphere, Climate 2 (COSMIC-2) radio occultation (RO) wet retrievals (temperature water vapor profiles) as input to Community Radiative Transfer Model (CRTM). ...

Upon coexpression with Erwinia geranylgeranyldiphosphate (GGDP) synthase in Escherichia coli, C(30) carotenoid synthase CrtM from Staphylococcus aureus produces novel carotenoids with the asymmetrical C(35) backbone. The products of condensation of farnesyldiphosphate and GDP, C(35) structures comprise 40 to 60% of total carotenoid accumulated. Carotene desaturases and carotene cyclases from C(...

Synthetic biology aspires to construct natural and non-natural pathways to useful compounds. However, pathways that rely on multiple promiscuous enzymes may branch, which might preclude selective production of the target compound. Here, we describe the assembly of a six-enzyme pathway in Escherichia coli for the synthesis of C50-astaxanthin, a non-natural purple carotenoid. We show that by judi...

To generate a random mutant library that is free from mutation at a particular amino acid residue, we replace the codon of interest with a detachable, short DNA sequence containing a BsaXI recognition site. After PCR mutagenesis, this sequence is removed and intramolecular ligation of the sequences flanking the insert regenerates the gene. The three-base cohesive ends for ligation correspond to...

The role of various gene loci was investigated in a family in which familial osteoarthritis (FOA), with onset at an early age, is transmitted as an autosomal dominant mendelian trait. The absence of clinical and radiographic signs of dysplasia and calcium pyrophosphate deposition disease (CPDD) indicates that the basic disease process in this family is osteoarthritis (OA). Genetic linkage analy...

A wood-based prepreg was formed using vacuum assisted resin transfer molding (VARTM) and a low viscosity thermoplastic resin. Wood strands were assembled to make porous mat for injection. The filled most of the cavities inside wood cells resulting in void volume fraction 7%. Young’s modulus strength saturated 38% 124% higher, respectively, than those prior infusion. Flat laminates produced by t...