The protein kinases and phosphatases in the signal transduction pathways play a vital role in mitogen activated protein (MAP) kinase signal transduction, tumorigenesis, apoptosis, and cancer metastasis. In this study, genetically engineered yeast strains were used as a model in the search for potential inhibitors against these signalling elements. Eleven species of medicinal plants were selecte...

With the development of high resolving mass spectrometers, metaproteomics evolved as a powerful tool to elucidate metabolic activity of microbial communities derived from full-scale biogas plants. Due to the vast complexity of these microbiomes, application of suitable fractionation methods are indispensable, but often turn out to be time and cost intense, depending on the method used for prote...

We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chlor...

Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative p...

The crude extract of a New Zealand ascidian displayed antitumor activity. Bioassay-directed fractionation yielded a novel alkaloid, coproverdine (1). The structure of 1 was assigned on the basis of detailed spectroscopic analysis. Coproverdine (1) was responsible for the antitumor activity of the crude extract.

This paper proposes a technique for optimizing crude cut points. Taguchi method is applied in the selection of the significant variables and their respective optimal levels for a fractionation process. The variables considered were the cut point temperatures of products, namely, naphtha, kerosene, light and heavy diesels, atmospheric gas oil and the residue. The variables were assigned lower an...

this experiment was conducted to evaluate the effects of different levels of crude protein and probiotic in diets on performance, egg quality  and blood biochemical and immunity parameters of laying hens. a completely randomized design with factorial method (2×4) was conducted in which two levels of crude protein (nrc and 10% lower than nrc recommendation) and 4 levels of probiotic (0, .025%, 0...

The nucleic acids of tubercle bacilli have formed the subject of several studies, among which may be mentioned those by Ruppel (l), Levene (2), and Johnson and his associates (3-5). The work of Mensel and Heidelberger (6) on the fractionation of the proteins of the tubercle bacillus revealed the presence of several protein fractions, rich in phosphorus and in purines, which appeared to be nucle...