نتایج جستجو برای: culture techniques
تعداد نتایج: 866537 فیلتر نتایج به سال:
This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.
Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ABH1 hydrophobin of Agaricus bisporus in A. niger, we showed that low producti...
هدف از انجام این مطالعه مقایسه دو روش mpn-pcr و mpn-culture در شمارش باکتری لیستریا مونوسیتوژنز تلقیح شده به شیر استریل می باشد. برای انجام این کار تعدادcell/ml 103 از باکتری لیستریا مونوسیتوژنز و نیز باکتری های زمینه ای مختلف به شیر استریل تلقیح شدند. پس از آماده سازی رقت های متوالی، از هر رقت در سه لوله حاوی محیط غنی کننده لیستریا تلقیح شد. پس از 48 ساعت گرمخانه گذاری، برای آزمایش mpn-cultu...
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...
We present a straightforward method to create spatial gradients of substrate bound protein for live cell studies using only mechanical parts. Protein concentration gradients on a micron scale can be fabricated in several minutes for a relatively low cost using a method that is generally applicable to any protein and substrate combination. We describe the details of the device construction, and ...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید