Two low structured mathematical models for fed-batch production of polyhydroxybutyrate and poly[hydroxybutyrate-co-hydroxyvalerate] by Cupriavidus necator DSM 545 on renewable substrates (glycerol and fatty acid methyl esters-FAME) combined with glucose and valeric acid, were established. The models were used for development/optimization of feeding strategies of carbon and nitrogen sources conc...

UNLABELLED The large legume genus Mimosa is known to be associated with both alphaproteobacterial and betaproteobacterial symbionts, depending on environment and plant taxonomy, e.g., Brazilian species are preferentially nodulated by Burkholderia, whereas those in Mexico are associated with alphaproteobacterial symbionts. Little is known, however, about the symbiotic preferences of Mimosa spp. ...

Molar growth yields were determined from chemostat cultures of Hydrogenomonas eutropha on succinate and on fumarate. The yields from culture on succinate were about 12 g higher than on fumarate. Assuming this difference to be equivalent to 1 molecule of adenosine triphosphate, it is concluded that the oxidation by oxygen of the Hydrogenomonas cytochrome b yields 1 molecule of adenosine triphosp...

Because of their lower environmental impact, biodegradable polymers such as polyhydroxyalkanoates (PHAs) produced within cultured bacteria represent promising alternatives to petroleum-based plastics. PHA production in flasks yielded optimal results with a carbon-to-nitrogen ratio 22. The 5-L scale experimental revealed that when glucose was used the carbon source, Cupriavidus necator could pro...

BACKGROUND Cupriavidus necator has attracted much attention as a platform for the production of polyhydroxyalkanoate (PHA) and other useful materials. Therefore, an appropriate modulation of gene expression is needed for producing the desired materials effectively. However, there is insufficient information on the genetic engineering techniques required for this in C. necator. RESULTS We foun...

A group of 20 bacterial strains was isolated from the rhizosphere of different agricultural plants growing in alkaline soils in the northeast of Mexico. The phylogenetic analysis of the 16S rRNA gene sequence from four strains showed that this novel group belonged to the Cupriavidus genus, with C. taiwanensis (∼98.9%) and C. necator (∼98.8%) as the closest species. However, DNA-DNA reassociatio...

Although PHAs are regarded as an effective substitute for conventional plastics for a number of medical and agricultural applications1 and food packaging2, their full-scale manufacturing is hampered by high production costs3. Factors affecting the cost of PHAs include raw materials availability, suitable process design, and downstream processing4,5. Since almost 50 % of the total production cos...