Effect of Cryopreservation Protocol on Post-Thaw Characteristics of Stallion Spermatozoa. (August 2009) Jose L. Salazar, B.S., Texas A&M University Chair of Advisory Committee: Dr. Dickson D. Varner Three ejaculates from each of eight stallions were initially centrifuged in INRA 96 extender and spermatozoal pellets were resuspended in a milk/egg yolk-based freezing extender or an egg yolk-based...

objective: adiponectin and its receptors (adipor1 and adipor2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. in this regard, we evaluated the correlation between sperm mrna abundance of adiponectin and its receptors, with sperm mot...

Objective Adiponectin and its receptors (AdipoR1 and AdipoR2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. Several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. In this regard, we evaluated the correlation between sperm mRNA abundance of adiponectin and its receptors, with sperm moti...

Background: Fertilization and fecundation obviously are the phenomenon in which several factors are involved. Sperm membranous proteins are a series of these important elements. CD9, an inner acrosomal membrane protein, is one of the tetraspanin members that involved in sperm-egg fusion. CD52, is a GPI anchor protein which is expressed in the epididymal cells and during passage of sperms throug...

Background: In mammalian spermatozoa, motility is one of the most important parameters for evaluating the quality of the semen. From a functional point of view, motility is strongly related to the spermatozoa’s ability to manage its energy status, Thus, modulation of energy status could be translated into significant changes in motility patterns. The aim of the present study was to evaluate xyl...

Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessmen...

The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 μg/ml of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to co...

Background Sperm quality, including semen volume, sperm count, concentration, and total progressive motility (collectively, “semen parameters”), has declined in the recent decades. Computer-assisted analysis (CASA) provides kinematic parameters, temporal trends of which remain unclear. Our objective is to examine trend both parameters Shanghai, China, years. Methods This retrospective study ana...

In the present study, we evaluated effect of various concentrations date palm pollen (DPP) aqueous extract on rabbit sperm during storage at 4°C for 48 h. Semen was collected from old and young bucks using an artificial vagina initially quality. The were diluted in Tris buffer supplemented with (20, 40, 80 mg/mL) DPP extract. extended samples stored Sperm motility motion kinetics assessed after...

Since all domestic turkeys are produced through artificial insemination, a measurable sperm characteristic that would be predictive of fertility would allow for the culling of poor males, resulting in improved reproductive efficiency. The sperm mobility test (SMT), which quantifies sperm penetration into an Accudenz solution, has been shown to correlate highly with fertilization potential of in...