Cisbio offers a comprehensive line of HTRF cytokine assays to investigate the functional response of immune cells. These versatile, homogeneous assays are suitable for a variety of sample types, such as cell culture supernatants, using immortalized cell lines or more physiological cell models such as PBMC. Blood Assay Solutions is specialized in the development of assays performed in whole bloo...

Abstract Background Advances in regenerative medicine technologies have been strongly proposed the management of thyroid diseases. Mechanistically, adoption bioengineering requires a scaffold that shares similar three-dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to native extracellular matrix (ECM). Methods 24 male New Zealand white rabbits were u...

Advanced cell culture techniques such as 3D bioprinting and hydrogel-based embedding harbor many new exciting opportunities to study cells in environments that closely recapitulate vivo conditions. Researchers often these using fluorescence microscopy visualize the protein association with objects within environment, yet quantification of concentration profiles microenvironment has remained elu...

The detection and quantification of equine cytokines has been hampered by the lack of antibodies for many years. With the development of antibody pairs for equine cytokines during the past years, the quantification of these essential regulators of the immune response became possible. After being successfully tested by enzyme-linked immunosorbent assays (ELISA), three of these anti-cytokine reag...

A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory respon...

The article describes a polymerase chain reaction (PCR) based method for semiquantitative assessment of mRNA expression for chemotactic cytokines in the lower respiratory tract cells. Cytokine mRNA was detected using PCR preceded by reverse transcription of mRNA into cDNA. The procedures of RNA extraction and of reverse transcription-PCR assay are described in the first part of the article. The...

Cytokine monitoring has attracted great attention due to its significance in the diagnosis and treatment of many diseases, such as tumors, microbial infections, immunological diseases. Enzyme-linked immunosorbent assay (ELISA) is one most popular methods cytokine detection, ascribing lavish signal amplification ELISA platform. In addition classical enzymes, other amplifiers fluorescent probes, ...

A prospective cross-sectional blinded study of 28 patients (21 male and 7 female patients; mean age, 44 years) with suspected active tuberculosis (TB) attending a TB and chest clinic is described. Blood was taken for immune cell enumeration, a whole-blood enzyme-linked immunosorbent assay (ELISA) for the detection of gamma interferon (IFN-gamma) by the QuantiFERON-TB Gold (QFT-G) assay, and int...