Plasmids and cloning cDNA for human USP4 and USP8 was a gift from Hidde Ploegh and cDNA for USP39 was a gift from R. Medema. USP4CD (aa 296-954) and the D1 fragment (aa 296-490) of human USP4, USP39CD (aa 219-565) and USP8CD (aa 771-1118) were cloned using ligation independent cloning into pET-46 Ek/LIC vector (Novagen). The D2 fragment (aa 766-932) of USP4 was cloned into the pET-NKI b/3C (Lun...

CD4-immunoglobulin G2 (CD4-IgG2) incorporates four copies of the D1D2 domains of CD4 into an antibody-like molecule that potently neutralizes primary human immunodeficiency virus type 1. Here electron microscopy was used to explore the structure and functional valence of CD4-IgG2 in complex with gp120. CD4-gamma2, a divalent CD4-immunoglobulin fusion protein, was evaluated in parallel. Whereas ...

mold isolated from pleural fluid and blood of the patient produced velvety, white colonies on Sabouraud dextrose agar (Figure, panel B). D1D2 rDNA sequenc-ing identified the mold as E. parva. Because we found no previous reports of E. parva disseminated infections, we sent the isolate to a reference laboratory (University of Alberta Microfungus Collection and Herbarium, Edmon-ton, Alberta, Cana...

The climbing perch, Anabas testudineus, is a nutritionally and economically significant food fish. present study reveals the first comprehensive description of life-history traits A. testudineus scooped up through different traditional fishing gears from July 2020 to December 2020. Among 120 collected specimens, smallest largest specimens were 8.5 cm–14.6 cm TL in Nilphamari Patuakhali, respect...

Abhängigkeit des pH-Wertes zu erstellen.

von Willebrand factor (VWF) is a multimeric plasma protein that mediates platelet adhesion to sites of vascular injury. The hemostatic function of VWF depends upon the formation of disulfide-linked multimers, which requires the VWF propeptide (D1D2 domains) and adjacent D'D3 domains. VWF multimer assembly occurs in the trans-Golgi at pH ~ 6.2 but not at pH 7.4, which suggests that protonation o...

Systemic fungal infections represent a major cause of morbidity and mortality in immunocompromised patients. The ever-increasing number of yeast species associated with human infections that are not covered by conventional identification kits, and the fact that moulds isolated from deep infections are frequently impossible to identify using classical methods due to lack of sporulation, has driv...

Let Fq be a finite field of cardinality q and characteristic p. Let P (x) be any one-variable Laurent polynomial over Fq of degree (d1, d2) respectively and p d1d2. For any fixed s ≥ 1 coprime to p, we prove that the q-adic Newton polygon of the L-functions of exponential sums of P (xs) has a tight lower bound which we call Hodge-Stickelberger polygon, depending only on the d1, d2, s and the re...