Koudela B., L. Steinhauser: Evaluation of Vitality of Sarcocysts in Beef by the DAPI Fluorescence Test. Acta vet. Brno, 53,1984: 193-197. Vitality of Sarcocystis sp. cystozoites in beef was evaluated by means of the DAPI fluorescence test. Results were verified by biological assays in definitive hosts. Sarcocysts remained vital in beef kept at refrigerator temperature over a period of 20 days a...

Time-resolved confocal microscopy has been applied to study the cytoplasm and nucleus region of a single live Chinese hamster ovary (CHO) cell. To select the cytoplasm and the nucleus region, two different fluorescent probes are used. A hydrophobic fluorescent dye, DCM, localizes preferentially in the cytoplasm region of a CHO cell. A DNA binding dye, DAPI, is found to be inside the nucleus of ...

Besides photometric cytometry (PC), fl uorescence microscopy combined with computerised image analysis, i.e. image cytometry (IC), offers an alternative tool for assessing genome size. These techniques allow direct visualization of hyphae and simultaneous measurement of nuclear fl uorescence intensity. We developed a simple method for quantitative evaluation of nuclear DNA in fungi using DAPI-I...

Cellular viability is usually determined by measuring the capacity of cells to exclude vital dyes such as 4',6-diamidino-2-phenylindole (DAPI), or by assessing nuclear morphology with chromatinophilic plasma membrane-permeant dyes, such as Hoechst 33342. However, a fraction of cells that exclude DAPI or exhibit normal nuclear morphology have already lost mitochondrial functions and/or manifest ...

Bacteria were either heat fixed on microscope slides or filtered with 0.2 micron-pore-size Nuclepore filters. The samples were stained with 4',6-diamidino-2-phenylindole (DAPI) for total staining and with polyvalent rabbit antibodies and fluorescein isothiocyanate-conjugated swine anti-rabbit antibodies for specific staining. By switching between two different optical filter packages in the mic...