A biore actor for the mechanical stimulation of silk based scaffolds has been developed. The bi oreactor simulates the natural movement of the anterior cruciate ligament by applying a biaxial movement on the scaffold. The silk scaffolds are prepared in a way that mimicks the mechanical properties (strength, stiffness) of natural anterior cruciate ligaments. The influence of degumming on the mec...

Lecitase Ultra lipase is an acidic lipase which exhibits maximal activity at pH=5.0. It takes part in the activity towards both phospholipid and triglyceride structures. When the temperature is over 40 °C, the phospholipase activity predominates, and the lipase activity is partly suppressed. It could be applied for the degumming of vegetable oil, and the phosphatides in the oil were easily conv...

BACKGROUND Enzymatic biodiesel is becoming an increasingly popular topic in bioenergy literature because of its potential to overcome the problems posed by chemical processes. However, the high cost of the enzymatic process still remains the main drawback for its industrial application, mostly because of the high price of refined oils. Unfortunately, low cost substrates, such as crude soybean o...

BACKGROUND Phospholipase A1 is an enzyme that hydrolyzes phospholipids at the sn-1 position. It has potential applications across diverse fields including food, pharmaceutical, and biofuel industries. Although there has been increasing interest in the use of phospholipase A1 for degumming of plant oils during biodiesel production, production of recombinant phospholipase A1 has been hampered by ...

Wild Silkmoth cocoons are difficult or impossible to reel under conditions that work well for cocoons of the Mulberry silkmoth, Bombyx mori . Here we report evidence that this is caused by mineral reinforcement of Wild Silkmoth cocoons and that washing these minerals out allows for the reeling of commercial lengths of good quality fibers with implications for the development of the "Wild Silk" ...

Silk fibroin is a protein with unique combination of properties and widely studied for biomedical applications. The extraction (degumming) from the silk filament impacts outcoming material. degumming can be conducted different procedures. Among them, most used procedure in research field alkali sodium carbonate (Na2CO3). In this study, by use statistical method, namely, design experiment (DOE),...