OBJECTIVES Odontoblasts are highly differentiated post-mitotic cells, which under pathological conditions such as carious lesions and dental injuries may degenerate and be replaced by other pulp cells. We have recently shown that this physiological event can be reproduced in an in vitro assay system, but is highly modified by the presence of unpolymerized resinous monomers. Our hypothesis was t...

OBJECTIVE The purpose of this in vitro study was to explore the radiopacity of composite resins and bonding materials using film and phosphor plates. METHODS Nine composite dental resin specimens and human tooth slices were exposed together with an aluminium stepwedge using dental film and phosphor plates. Eight dentin bonding specimens were prepared and exposed in a similar manner. Their rad...

OBJECTIVE The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells. STUDY DESIGN Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A co...

The purpose of this study was to compare the cytotoxicity of five one-step dentin-bonding agents on human dental pulp and odontoblast-like cells (MDPC-23). Photopolymerized and unpolymerized samples of these dentin-bonding agents were prepared and incubated with dental pulp or MDPC-23 cells. After 24 or 72 h of incubation, the number of unstained cells with trypan blue was counted. The staining...

background: bleaching the discoloured teeth may affect the tooth/composite interface. the aim of this in vitro experimental study was to evaluate the effect of vital tooth bleaching on microleakage of existent class v composite resin restorations bonded with three dental bonding agents.     methods: class v cavities were prepared on buccal surfaces of 72 intact, extracted human anterior teeth w...