C3H mice were immunized with MH134 tumors modified with two different chemicals, DEPC and TNBS, and the tumor-neutralizing activities of the spleen cells were examined after separating the cells by a Nylon-wool column, plastic dish, or treatment with specific antibodies (anti-mouse IgG and anti-mouse theta sera). The effector cells induced in mice by immunization with TNBS-treated tumors were T...

Materials. The HPLC-purified microRNAs and PAGE-purified DNA oligonucleotides (Table S1) were synthesized by TaKaRa Biotechnology Co., Ltd. (Dalian, China). The deoxynucleotide solution mixture (dNTPs), RNase inhibitor and diethyl pyrocarbonate (DEPC)-treated water were obtained from TaKaRa Bio. Inc. (Dalian, China). The Vent (exo-) DNA polymerase and nicking endonuclease Nt.BstNBI were purchas...

In the title compound, [Li(C(27)H(23)N(2))(C(4)H(10)O)], the Li atom possesses a nearly planar trigonal coordination environment (assuming the cyclo-penta-dienyl ring of the indenyl group occupies one coordination place). The diethyl ether ligand adopts a nearly planar W-type conformation.

In the title mol-ecule, C(24)H(31)N(5)O(6), the two ethyl fragments are each disordered over two conformations [occupancy ratios 0.58 (13)/0.42 (13) and 0.56 (12)/0.44 (12)]. The crystal packing exhibits inter-molecular non-classical C-H⋯O hydrogen bonds and π-π inter-actions between benzene rings [centroid-centroid distances = 3.836 (5) Å].

reacted with other reactive groups in the protein. However, it would appear that, whatever other reactions may have occurred, the increase in specifically monitors the extent of modification of histidine residues only. Thus diethyl pyrocarbonate appears to have reacted with pig pepsinogen to modify, at least, all three histidine residues in the protein. Once again, therefore, the potential acti...

In the title compound, C(24)H(29)Br(2)N(5)O(6), a glycoluril derivative, the 1,4-dibromo-benzene ring is fused to the seven-membered ring of the glycoluril unit containing two N atoms. The two five-membered rings in the glycoluril unit are approximately planar and the dihedral angle between them is 69.8 (2)°. The six-membered ring containing three N atoms adopts a chair conformation. The crysta...

In the title compound, C(20)H(21)N(2)O(5)PS, the indole ring is essentially planar, with a maximum deviation of -0.0083 (18) Å. The methyl C atom of the methyl-phospho-nate group and the S atom lie 0.104 (2) and -0.2158 (6) Å, respectively, from the indole mean plane. The sulfonyl-bound phenyl ring is almost perpendicular to the indole ring system, with a dihedral angle of 82.30 (8)°. The ethyl...

Kinetic studies and chemical modification studies using diethylpyrocarbonate and iodoacetate were performed on malate dehydrogenase isolated from Escherichia coli. Product inhibition experiments indicate that this enzyme follows an ordered Bi Bi kinetic mechanism, similar to other dehydrogenases, while log V/K profiles reveal that one ionizing group with a pKa between 7.8 and 8.7 acts as a gene...

The role of the four histidine residues in receptor binding and activity of mouse nerve growth factor (NGF) was investigated using both site-directed mutagenesis and chemical modification with diethyl pyrocarbonate. Replacement of His-75 or His-84 with alanine resulted in decreased biological activity and decreased affinity for p140(trkA); however, with H75A only, a 5-fold increased affinity to...