Yeast glutamate-cr-ketoadipate transaminase activity exhibited differences in differential heat inactivation, pH dependence, response to pyridoxal phosphate, ammonium sulfate fractionation, diethylaminoethyl cellulose chromatography, and mutational independence from other known transaminases. Both yeast glutamate-ol-ketoadipate transaminase and glutamate-pyruvate transaminase were found to be p...

We have compared two methods for separating the isoenzymes of creatine kinase in serum and measuring their activity: chromatography on diethylaminoethyl-Sephadex A-50, with both continuous and discontinuous gradient elution, and electrophoresis on cellulose acetate. Results by continuous and discontinuous gradient elution correlated well both for tissue extracts and sera. Electrophoresis on cel...

Chitosan is prepared by the deacetylation of chitin, the second-most abundant biopolymer in nature, and has applicability in the removal of dyes, heavy metals and radioactive waste for pollution control. In weight-reduction remedies, chitosan is used to form hydrogels with lipids and to depress the intestinal absorption of lipids. In this study, an experimental method was implemented to simulat...

Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis, H(37)Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a(1), is not antigenic. The slower migrating protein, des...

By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the A component of the group D lysin produced by Streptococcus zymogenes has been purified to a state of apparent homogeneity when determined by the techniques of anionic and cationic disc gel electrophoresis...

Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of these activities have been mapped and are widely separated from each other on th...

Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains. Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88:216-219. 1964.-A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified dieth...

The B protein, a subunit of lactose synthetase (EC 2.4.1. c), was crystallized from bovine skim milk and bovine mammary tissue. The B protein was identified as cu-lactalbumin, based on the following criteria: substitution in the enxymic rate assays, spectra, immunological titrations, amino acid composition, mobility on starch gel electrophoresis, molecular weight, and cochromatography on diethy...

Nuclei were isolated by hypotonie shock and homogenization from freshly drawn, nonstimulated leukemic leukocytes and were pulse labeled with deoxythymidine triphosphate-3H. Labeled oligodeoxyribonucleotides were separated from the bulk of DNA and proteins by membrane cone filtration and were further purified by alkaline hy drolysis and diethylaminoethyl cellulose chromatography. In parallel exp...