Studyon cultivation of Polyporus squamosusin aqueoustwo-phase systemconsisting of7% (wi w) polyethylene glycol (M" 4,000) and 7.5% dextran (M" 40,000) wasconducted. Fungusgrowth wasrestricted in bottom phase, while thepresence of endo-pectinase activity in one of thephases waschangeable duringthecultivation. All endo-pectinase activity excreted on the 3,d dayofcultivation wasseparated in the to...

This investigation was concerning with isolation and identification of fungal species from soil in upper Egypt (Assiut). Thirty fungal species belonging to eighteen genera were collected and screened for their ability to produce cellulase and pectinase enzymes. The most potent was Mucor fuscus (MS22) and was allowed to grow on industrial cellulose and pectin under different cultural and nutriti...

Softening, which accompanies ripening fruits is also to a large extent, responsible for the damage that occurs during handling and shipping. The effects of 1-Methylcyclopropene (1-MCP) application at different ripening stages (mature green, breaker, breaker + 2 days and breaker + 4 days) on cellulase and pectinase activities of cherry tomato fruit (Lycopersicon esculentum Mill. var. cerasiforme...

The effect of ultrafiltered xylanase-pectinase concoction produced simultaneously by a bacterial isolate using agro-waste-based media was assessed in prebleaching of plywood waste pulp. Ultrafiltered enzymes caused 12.5% reduction in kappa number at reduced enzyme dose of xylanase-pectinase (4.0-0.8 IU) per gram of pulp under optimized conditions at pH 8.5, temperature 55 °C, and treatment peri...

The production of a notable and highly effective pectinase and cellulase, by the commercial baker’s yeast Saccharomyces cervisiae utilizing potato processing wastes, was achieved in 5-day solid state fermentation (SSF) cultures, at temperature 25 °C, pH range 4.0-5.0 and additive of ferric chloride. Pectinase and cellulase activities were stimulated by using potato wastes supplemented with urea...

A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, t...

Cutinase from Thermobifida fusca is thermally stable and has potential application in the bioscouring of cotton in the textile industry. In the present study, the carbohydrate-binding modules (CBMs) from T. fusca cellulase Cel6A (CBM(Cel6A)) and Cellulomonas fimi cellulase CenA (CBM(CenA)) were fused, separately, to the carboxyl terminus of T. fusca cutinase. Both fusion enzymes, cutinase-CBM(C...

Landoltia punctata, a widely distributed duckweed strain with the ability to accumulate starch, was used as a novel feedstock for bioethanol production by Saccharomyces cerevisiae. To improve ethanol production, pectinase pretreatment was used to release much more glucose from L. punctata mash and the pretreatment conditions (enzyme loading, temperature and pretreatment time) for the duckweed w...

BACKGROUND The use of immobilized enzymes for catalyzing various biotransformations is now a widely used approach. In recent years, cross-linked enzyme aggregates (CLEAs) have emerged as a novel and versatile biocatalyst design. The present work deals with the preparation of a CLEA from a commercial preparation, Pectinex Ultra SP-L, which contains pectinase, xylanase and cellulase activities. T...