methods this prospective observational study was conducted in a tertiary level neonatal intensive care unit, princess esra hospital, deccan college of medical sciences, hyderabad, telangana, india. consecutive intramural and extramural neonates admitted to neonatal intensive care unit with the rh-negative mother’s blood group and hyperbilirubinemia were enrolled. neonates born to rh+ve mothers ...

background: delay in diagnosis and treatment of perforated appendicitis may cause life-threatening complications. the aim of this study was to determine and compare pre-operative total and direct bilirubin levels in cases of simple and perforated acute appendicitis in order to improve the clinical decision making.   methods: this prospective observational study included eighty patients who unde...

OBJECTIVES Cigarette smoking is a modifiable risk factor for cardiovascular disease. Bilirubin is a potent antioxidant and its concentration decreases in smokers. However, studies about the association between cigarette smoking and bilirubin are scarce and most are limited to total bilirubin. Additionally, bilirubin is highly related to hemoglobin. Therefore, this study evaluates the associatio...

We describe an improved colorimetric method for assays of total and direct bilirubin in serum. Bilirubin reacts with diazotized sulfanilic acid in an acidic medium to form a blue azopigment. Total bilirubin is assayed in the presence of reaction accelerators (caffeine, urea, and citric acid), direct bilirubin in their absence. The azo compound so formed is read at the same wavelength (570 nm) i...

We determined total bilirubin, "direct" and "indirect" bilirubin fractions, and, in parallel, using Ektachem slides, the total, conjugated, unconjugated, and delta bilirubin contents for specimens from 88 adult inpatients at various times during hospitalization in a tertiary-care facility. We wanted to see if the results correlated with the patients' diagnoses, based on a review of medical reco...

The yellow pigments observed in the ocular humors of premature infants were identified as the plasma pigments bilirubin and oxyhemoglobin. The blood/vitreous humor barrier to bilirubin was estimated to be (1/0.11) for total bilirubin and (1/0.25) for direct-acting bilirubin. Hall method histochemical staining for bilirubin in sections of premature infant eyes was highly positive for the vitreou...

background: transcutaneous bilirubinometry (tcb) is a simple method for estimating bilirubin levels in neonates. this method is noninvasive, quick, and painless. we aimed to compare serum and cutaneous bilirubin measurements in term neonates.method: in this descriptive cross-sectional study, 200 neonates with icter with birth weights of at least 2500 grams were studied. tcb was measured using a...

Objective: Recently, the role of hemolysis in the pathophysiology of hyperbilirubinemia in glucose-6phosphate dehydrogenase  (G6PD)  deficient  neonates  has  been  questioned  and  decreased  bilirubin conjugation has been suggested. We conducted a study to evaluate the effect of hemolysis on these neonates at the 17-Shahrivar Children Hospital in Rasht.  Methods: In  this  cross-sectional  s...

BACKGROUND Serum bilirubin may have potent antioxidant and cytoprotective effects. Serum bilirubin levels are inversely associated with several cardiovascular and metabolic endpoints, but their association with nonalcoholic fatty liver disease (NAFLD) has not been investigated except for a single cross-sectional study in a pediatric population. We assessed the prospective association between se...