PURPOSE The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. METHODS In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified ...

BACKGROUND Controlled-rate freezing of ovarian cortical tissue for preservation of fertility among young women facing chemo- or radio-therapy is a widely accepted procedure. To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing. METHODS Ovarian tissue from 20 women, donated...

Ovulated mouse oocytes denuded of their cumulus cells, were vitrified in a solution containing 7 M ethylene glycol as the sole cryoprotectant using one or two steps of exposure before vitrification and were diluted in 1 M sucrose solution in 5 or 10 min after warming. The results proved that the viability of oocytes are detrimentally affected by exposure to the vitrification solution even witho...

Background: Many sperm characteristics including, motility and morphology are important for normal spermatozoa- oocyte intraction. These features may be altered during vitrification. Motile sperm organelle morphology examination (MSOME) is an unstained real time high magnification technique that analysis viable sperm morphology instantly. This study aimed to investigate the influence of vitrifi...

This study was conducted to evaluate the effect of plant growth regulators on in vitro shoot multiplication, vitrification and rooting of two carnation cultivars (Eskimo Mogr and Innove Orange Bogr). Isolated axillary buds were cultured on MS medium supplemented with different levels of Benzyl amino purine (BAP) or kinetin (Kin) in combination with 0.2 mg/l NAA and shoot multiplication and vitr...

Vitrification is a powerful tool for the efficient production of offspring derived from cryopreserved oocytes or embryos in mammalian species including domestic animals. Genome editing technologies such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas)9 are now available even for domestic s...

This paper reports studies on the effects of re-vitrification by the CPS (Closed Pulled Straw) method on the development of 4-cell stage mouse embryos. The procedure involved culturing 2-cell mouse embryos in G-1 medium until the 4-cell stage followed by the division of the normal 4-cell stage embryos into a control group (non-vitrified) and two experimental subgroups (vitrified and re-vitrifie...

Type I collagen membranes with tailored fibril nanoarchitectures were fabricated through a vitrification processing, which mimicked, to a degree, the collagen maturation process of corneal stromal extracellular matrix in vivo. Vitrification was performed at a controlled temperature of either 5 °C or 39 °C at a constant relative humidity of 40% for various time periods from 0.5 wk up to 8 wk. Du...

BACKGROUND Ovarian tissue cryopreservation may be a potential method of preserving fertility in women who have experienced gonadotoxic treatments. To improve the efficiency of existing cryopreservation, we developed a practical and convenient vitrification method named needle immersed vitrification (NIV), which required a less concentrated and minimum volume of vitrification solution. METHODS...