With the discovery of newer antifungal agents, emergence of resistance among Aspergillus, is a major problem. In the present study, 25 different clinical isolates of Aspergillus flavus were screened for their susceptibility against five common antifungal agents by broth dilution and disc diffusion method with the aim to evaluate both the methods for their suitability to adapt in clinical labora...

OBJECTIVE To find out the frequency of low-level quinolone-resistance in Multi-Drug Resistant (MDR) typhoid using nalidixic acid screening disc. STUDY DESIGN Descriptive study. PLACE AND DURATION OF STUDY Armed Forces Institute of Pathology, Rawalpindi, from January 2005 to December 2005. MATERIALS AND METHODS Blood was obtained from suspected cases of typhoid fever and cultured in to Bac...

Methanolic extract of Elephantopus scaber Linn was investigated for its antibacterial activity against Staphylococcus aureus (NCIM - 2079), Escherichia coli (NCIM-2067), Bacillus subtilis (NCIM-2063), Pseudomonas aeruginosa (NCIM-2036), Proteus vulgaris (NCIM-2027) at 100 μg/disc by using disc diffusion method. The extract showed significant anti bacterial activity and were compared to chloramp...

PURPOSE The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance was determined by three different methods (disc-diffusion, Vitek 2 and E tes...

Objectives: Grewia paniculata (Family: Malvaceae) has been used to treat inflammation, respiratory disorders and fever. It is additionally employed for other health conditions including colds, diarrhea and as an insecticide in Bangladesh. The aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of Grewia paniculata. Materials and Methods: ...

K.E.M. Hospital, Parel, Mumbai-400012. Abstract Due to cost and complexity, reference broth microdilution antifungal susceptibility method is not feasible in resource constraint setting. Therefore here is a need to develop a cost effective method which would help in simultaneous rapid identification of Candida species and performance of disc diffusion susceptibility testing. Comparison of resul...

A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with th...

BACKGROUND AND OBJECTIVES Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also important. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastit...