PURPOSE To analyze the influence of time of storage, the presence of albumin at physiological concentrations, and the perfusion with anisosmotic media on the aqueous humor outflow facility (C) of isolated bovine anterior segments (AS). METHODS Anterior segments dissected from cow eyes were perfused at a constant pressure of 10 mm Hg with Dulbecco's modified Eagle's medium (DMEM; osmolality 30...

After cells attach the substrata, cells begin to spread and form an organized actin cytoskeleton and complex transmembrane signaling regions, then cells start to express their functions, such as proliferation, differentiation and so on. Therefore, cell adhesion is very important. Recently, a number of studies have investigated cell adhesion from the viewpoint of biochemistry, materials, and mec...

Vaccinia virus (VACV) is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits...

Though the instability of polyphenols in cell culture experiment has been investigated previously, underlying mechanism is not completely clear yet. Therefore, this study, stability epigallocatechin gallate (EGCG) medium DMEM was at 4 °C and 37 via UPLC-MS-MS analysis followed by determination antioxidant capacity EGCG. EGCG instable formed various degradation products derived from its dimer wi...

In this paper, the stability of magnesium-doped hydroxyapatite/chitosan (MHC) suspension obtained with sol-gel approach was evaluated using nondestructive ultrasound measurements. The MHC coatings by spin-coating technique were characterized before and after immersion for 7 14 days, respectively, in Dulbecco’s modified eagle medium (DMEM) scanning electron microscopy, equipped an EDAX detector....

PURPOSE The retinal pigment epithelium (RPE) in vivo is known to have polarized membrane domains that are essential for its normal function. Unless the proper cell culture conditions are used, these polarized features are often lost. In the past, the use of Chee's Essential Medium (CEM) in our RPE cultures has produced functional polarity of the cell monolayers. Unfortunately, except by custom ...

Supplementary Experimental Procedures Isolation, culture, and transfection of primary mammary epithelial organoids Primary mammary epithelial organoids were prepared from 8-week-old CD1 mice (Charles River) as previously described (Nelson et al., 2006). Inguinal glands were removed aseptically, minced with razor blades, and incubated with agitation (350 rpm) at 37°C in DMEM/F12 supplemented wit...

• Porous BPS hydrogel was fabricated. fast biomineralized using urease. The bone-like apatite formed on hydrogels. cytocompatibility of biomimeralized hydrogels improved. development with excellent is great importance for tissue engineering applications. Here, porous polyvinyl alcohol/alginate/biphasic calcium phosphate (BPS) were fabricated via chemical and physical crosslinking methods, the i...

The purpose of this investigation was to re-evaluate the neurotrophic effect of GPI-1046 on neurite outgrowth in vitro. GPI-1046 was synthesized and identified with mass spectrometry, nuclear magnetic resonance and elemental analysis. Chicken dorsal root ganglions (DRGs) were removed and divided into three groups: (1) The DRGs were cultured in DMEM containing different concentrations of GPI-104...