Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile donors comparing the sperm chromatin structure assay (SCSA), TdT-mediated-dUTP nick end labeling (TUNEL), the sperm chromatin dispersion (SCD) test, and acridine orange staining technique (AOT). The TUNEL...

BACKGROUND We assessed sperm DNA fragmentation index (DFI) in cancer patients before and after treatment to evaluate if sperm DNA integrity is compromised by cancer itself or its treatment. METHODS In a prospective study, DFI was assessed in 127 patients diagnosed with testicular germ cell tumours (TGCT), Hodgkin's lymphoma (HL), non-Hodgkin's lymphoma (NHL) and various malignancies. The seve...

OBJECTIVE To evaluate whether increasing antioxidant intake in men with high levels of DNA damage or lipid peroxidation improves gestational results in couples with history of recurrent embryo loss. DESIGN Descriptive study (case series). SETTING Early recurrent embryo loss program at the University of Antioquia, Medellín, Colombia. PATIENT(S) Seventeen men whose spouses had a history of ...

BACKGROUND Many factors may lead to sperm DNA damage. However, it is little known that the correlations of sperm DNA damage with obesity-associated markers, and reproductive hormones and lipids levels in serum and seminal plasma. METHODS In our prospective study, a total of 1 010 subfertile men, aged from 18 to 50 years old, were enrolled from August 2012 through June 2015. Their obesity-asso...

BACKGROUND The sperm chromatin structure assay (SCSA) has been suggested as a predictor of fertility in vivo as well as in vitro. The available data however, have been based on limited numbers of treatments. We aimed to define the clinical role of SCSA in assisted reproduction. METHODS A total of 998 cycles [387 intrauterine insemination (IUI), 388 IVF and 223 ICSI] from 637 couples were incl...

ObjectiveTo evaluate Deoxyribonucleic acid (DNA) methylation patterns in sperm from men with differential levels of DNA fragmentation index (DFI).DesignProspective study.SettingUniversity-affiliated reproductive medicine center.Patient(s)A total 278 male patients consulting for couple infertility were recruited the First Affiliated Hospital Wenzhou Medical University.Intervention(s)None.Main Ou...

Background: To establish importance of sperm DNA assessment in resolving the unexplained infertility problem. Materials and Methods: This article is based on the careful observation of texts and papers from 2000-2014 about the DNA damage assessment as a significant parameter of sperm function test. Results: Acidic Aniline blue, Sperm Chromatin Dispersion test(SCD), Malondialdehyde concentration...

BACKGROUND This case-control study was designed with the aim of evaluating the role of sperm, oxidative stress and DNA damage in idiopathic recurrent pregnancy loss (iRPL). This pilot study is the first study done on the Indian population which reports the association between DFI, TAC and ROS in couples experiencing iRSA. METHODS Twenty infertile men with a history of iRPL and 20 fertile cont...

Oxidative stress is detrimental to sperm function and a significant factor in the etiology of male infertility. This report examines the association between dietary and supplementary intake of the antioxidants vitamin C, vitamin E, and beta-carotene and sperm chromatin integrity. Eighty-seven healthy male volunteers donated semen samples, completed food-frequency questionnaires, and provided in...