Cell free fetal DNA (cff DNA) is apoptotic that shed from placenta and flows in maternal blood. This can be used as an indicative biomarker to rule out pregnancy associated complications. The objective of this study investigate the correlation concentration cff with intrauterine deaths. RHD gene (Rh blood group, D antigen) was marker for confirmation GAPDH (Glyceraldehyde-3-phosphate dehydrogen...

The analysis of environmental DNA (eDNA) by high throughput sequencing (HTS) is proving to be a promising tool for freshwater fish biodiversity assessment in Europe within the Water Framework Directive (WFD, 2000/60/EC), especially large rivers and lakes where current monitoring techniques have known shortcomings. These new biomonitoring methods based on eDNA show several advantages compared cl...

A polymorphic DNA marker has been found genetically linked, in a set of 39 human families, to an autosomal recessive gene that causes cystic fibrosis (CF), a disease affecting one in 2000 Caucasian children. The DNA marker (called D0CRI-917) is also linked to the PON locus, which by independent evidence is linked to the CF locus. The best estimates of the genetic distances are 5 centimorgans be...

DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100-1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA. Target DNA fragments were amplified using T...

InDel (insertion deletion length polymorphism) markers is designed for PCR detection based on the Insertion or of DNA fragments different sizes according to sequence a locus between individuals related species, which more and applied in crop genetic research due its rich polymorphism, operational simplicity reliability results. In this study, Qinyou 88 parents were analyzed through marker, four...

The identification of molecular markers linked to leaf curl virus (CLCuV) disease resistance in cotton has the potential to improve both the efficiency and the efficacy of selection in cotton breeding programs. Genetic analysis suggested that CLCuV resistance is controlled by a single dominant gene. In this study, an interspecific F2 population derived from a cross of Gossypium barbadense and G...

Plant genetic engineering has, until now, relied on the incorporation of foreign DNA into plant genomes. Public concern about the extent to which transgenic crops differ from their traditionally bred counterparts has resulted in molecular strategies and gene choices that limit, but not eliminate, the introduction of foreign DNA. Here, we demonstrate that a plant-derived (P-) DNA fragment can be...