background: colorectal cancer, the third most common type of cancer is a major cause of mortality worldwide. if colorectal cancer is detected at the early stages, the 5-year survival rate is 90%. meis1 homeobox gene is implicated in numerous solid tumors and hematological malignancies. therefore, the present study aims to investigate the methylation status of the meis1 gene in colorectal cancer...

Background: Ras-associated domain family 1 (RASSF1A) and hypermethylated in cancer (HIC1) genes are methylated more frequently in breast cancer. Genetic factors that alter the DNA methylation levels in normal and tumor tissues could therefore influence the susceptibility to this tumor phenotype. Objective: We determined the frequency of aberrant methylation of HIC1 and RASSF1A gene promoters an...

dna methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. to assess dna methylation pattern in different developmental stages of aeluropus littoralis, methylation sensitive amplified polymorphism (msap) was used. methylation and demethylation status at the ccgg recognition site were tracked ...

Promoter methylation is one of the main epigenetic mechanisms that lead to the inactivation of tumor suppressor genes during carcinogenesis. Due to the reversible nature of DNA methylation, many studies have been performed to correct theses epigenetic defects by inhibiting DNA methyltransferases (DNMTs). In this case novel therapeutics especially siRNA oligonucleotides have been used to specifi...

Background: Because of the protection of surplus embryos, Cryopreservation is usually used in ART. It is not clear, the vitrified-warmed embryos that have normal morphology, be normal in genetic level, too. DNA methylation of gene regulatory regions can causes inhibition of gene expression. We study effect of vitrification method of cryopreservation on DNA methylation and gene expression level ...

The epigenetic alterations like histone modifications , DNA methylation and others remarkable categories  including nucleosome remodeling and RNA mediated targeting have been strongly investigated  recently .In this way , beside the notable importance of DNA methylation ,the histone modifications are the most important issues in the  tumorogenesis and cancer progression. Moreover...

objective: zoledronic acid as a main treatment for osteoporosis has an important role in differentiation of mesenchymal stem cells. however, mechanism of osteoblastic differentiation induction by zoledronic acid is not well understood until now. in this research we evaluate zoledronic acid effect on methylation status of runx2 and dlx5 promoters. materials and methods: after isolation and expa...

asthma is a complex, heterogeneous and chronic airway inflammatory disease with different clinical phenotypes caused by diverse triggers and pathophysiological mechanisms. asthma heritability has been established in many genetic studies but it is evident that only genetic elements are not responsible for the development of asthma. increasing rate of asthma incidence during past decades has impl...