نتایج جستجو برای: dna size marker
تعداد نتایج: 1152875 فیلتر نتایج به سال:
Glossary Behavioral genetics The study of the contribution of genetics to behavior, the identification of genes involved, and the relationship between genes and environment. DNA profile A series of numbers recording the size of marker sections of DNA at particular points. Markers are repeated short sequences of DNA that vary in length between individuals. DNA profiles for forensic purposes may ...
We describe a simple and cost-effective method for the synthesis of an internal fluorescently labeled DNA standard for fragment sizing using an automated DNA sequencer. A set of primer pairs labeled with ROX was developed to amplify 12 DNA fragments, 58-417 bp, derived from a conserved region of plant chloroplast DNA. These amplified fragments were mixed together, constituting a fluorescent int...
background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...
Methods for automated detection of DNA restriction fragments resolved on agarose fingerprinting gels are described. We present a mathematical model for the location and shape of the restriction fragments as a function of fragment size, with model parameters determined empirically from marker lanes containing molecular size standards. Automated identification of restriction fragments involves se...
INTRODUCTION In most cases of cervical cancers, HPV DNA is integrated into the genome of carcinoma cells. This mutational insertion constitutes a highly specific molecular marker of tumor DNA for every patient. Circulating tumor DNA (ctDNA) is an emerging marker of tumor dynamics which detection requires specific molecular motif. To determine whether the sequence of the cell-viral junction coul...
( a ) Autoradiograph of RNA species which arise in a transcription reaction. Nomenclature, fragment sizes and experimental conditions are as in 141. RNA circles are arrowed wth their tentative sizes in nucleotides. Precursor (p), ribozyme (IVS), ligated exons (SE3E) and precursor in which the downstream exon has been hydrolysed (5EI) are depicted. ( b ) Autoradiograph of radiolabelled amplified...
In H. polymorpha, there is still a lack of a highly efficient gene disruption method. To help address this issue, we presented a simple and efficient method for both single and multiple gene disruptions in H. polymorpha. The knockout system combined a variation of sticky-end polymerase chain reaction method (SEP), split marker deletion method, co-transformation of single-stranded DNA and mutant...
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