Donor corneas with narrow scleral rims are often disqualified for Descemet's Stripping Automated Endothelial Keratoplasty (DSAEK), mainly because of fluid leak and low pressure when they are mounted onto an artificial anterior chamber (AAC). This report describes a novel method to tight-lock a donor cornea with a narrow scleral rim so that microkeratome cutting is possible, allowing a DSAEK pro...

PURPOSE To examine the role of the CD28-CD80-CD86 pathway of T-lymphocyte costimulation in corneal allograft rejection and the effect of blockade of that pathway on graft survival. METHODS Kinetics of CD80 and CD86 expression in the cornea and draining lymph nodes were examined by RT-PCR and immunohistochemistry in untreated allograft recipients in a high-responder rat model. The effect of bl...

Posterior folds in human donor corneas stored as whole eyes in moist chambers, or isolated in McCarey-Kaufman medium at 4 degrees C, have been studied by light and scanning electron microscopy to determine whether or not endothelial cells on the folds are damaged. Extensive, often irreversible cell damage was found associated with most of the folds. Hence posterior folds in donor corneas are of...

PURPOSE To determine the extent to which CD95 ligand (CD95L) expressed on corneal epithelium and endothelium influences survival of cornea grafts placed orthotopically and heterotopically in the anterior chamber (AC), an immune-privileged site. METHODS Corneas from eyes of C57BL/6 (B6) and B6.gld (CD95L deficient) mice were (1) rendered into small full-thickness fragments, with or without an ...

purpose: optisol-gs is still the most popular cold storage medium used in the united states for short term preservation of donated corneas. cebi medium is a new cold storage medium made by the central eye bank of iran for donor cornea storage. this study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in optisol-gs versus in the cebi medium. m...