We investigated the erythrocyte membrane proteins of two patients with congenital hemolytic anemia due to increased permeability of the erythrocyte membrane to Na and K (hereditary stomatocytosis and cryohydrocytosis). One-dimensional sodium dodecyl sulfate (SDS) gel electrophoresis resolved the band 7 erythrocyte membrane proteins into three components with approximate molecular weights of 30,...

The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described. The following related problems have been considered: covalent and non-covalent association of protein subunits in multiple oligomeric structures; chromosomal locations of genes encoding seed proteins; quantita...

Two-dimensional electrophoresis (2-DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sort’s protein according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the se...

gamma camera is an important apparatus in nuclear medicine imaging. its detection part is consists of a scintillation detector with aheavy collimator. substitution of semiconductor detectors instead of scintillator in these cameras has been effectively studied. in this study, it is aimed to introduce a new design of p-n semiconductor detector array for nuclear medicine imaging. a p-n semiconduc...

isolation of cell free fetal dna (cffdna) from maternal serum usually leads to very low concentrations of dna impeding further resolving through conventional methods of electrophoresis. although several protocols have been described for capillary electrophoresis (ce) of double stranded dna, they usually need using special polymers or coated capillaries which degrade over time. herein, we propos...

Phosphorus (P) is an important mineral nutrient for plant growth and development. Overexpressing AtWRKY6 (35S:WRKY6-9) was more sensitive and wrky6 (wrky6-1) was more resistant under low Pi conditions. To better understand the function of AtWRKY6 under low phosphate stress conditions, we applied two-dimensional gel electrophoresis (2-DE) to analyse differentially expressed proteins in the shoot...

We introduce a dihomotopy invariant of a po-space in dimension 2, its dihomotopy double category. This is a generalisation both of the fundamental category of a pospace, and of the double homotopy groupoid of a topological space. We conjecture a van Kampen theorem for the dihomotopy double category, thus making available a tool for calculations.

Background: The simultaneous existence of two different abnormalities in a gene is referred to double heterozygosity. The most common form of it is the association of thalassemia and sickle cell disease, being termed sickle cell thalassemia. The aim of this research was to study all of the hemoglobinopathies in thalassemia major patients in Lorestan Province. Materials and methods: This st...