We performed a comparative analysis between Roche Amplicor HIV-1 DNA test and CAPTAQ assay for the detection of HIV in 830 dried blood spot (DBS) pediatric samples collected in Mozambique. Our results demonstrated no statistical difference between these assays. The CAPTAQ assay approached nearly 100% repeatability/accuracy. The increased throughput of testing with minimal operator interference ...

The relative concentrations of IgM and IgG antibodies (Ab) to Schistosoma mansoni soluble egg antigen (SEA) were evaluated in paired samples of venous blood sera and buffer-eluates of capillary blood drops dried on filter papers. The samples were obtained from school children at early and chronic stages of schistosomiasis diagnosed on the basis of history, clinical symptomatology and parasitolo...

We established the Bio-repository at the National Cancer Center Hospital in October 2002. The main purpose of this article is to show the importance and usefulness of a bio-repository of post-clinical test samples not only for translational cancer research but also for routine clinical oncology by introducing the experience of setting up such a facility. Our basic concept of a post-clinical tes...

Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-...

The present paper reports evidence of an immunological relationship between one variety or type of the yeast Saccharomyces cerevisi~e and the Type II variety of Diplococcus pn,umoni~e (Pneumococcus). The immunological interrelationships among the different yeasts have never been well established, but it is certain that all of the yeasts included by the species term Saccharomyces cerevisi~ are n...

An indirect (I) enzyme-linked immunosorbent assay (ELISA) and a competitive (C) ELISA, using a group-specific monoclonal antibody against bluetongue virus (BTV), are described for the detection of antibodies to BTV in cattle and sheep sera. The performance of these assays in detecting anti-BTV antibody in sequential serum samples and eluates from whole blood (WB) dried on filter paper from thre...

Traditional newborn dried blood spot screening (NDBS) has expanded in recent years to include upwards of 50 different conditions in many states. Once the screening tests are complete, the residual blood specimens (RBSs) that remain are most often available for quality assurance and public health research. The articles by Botkin et al1 and Bombard et al2 contained in this issue of Pediatrics inv...

background: the aim of this study was using elisa for detection of anti- schistosoma haemato­bium antibodies in both sera and urine of patients with urinary schistosomiasis. methods: thirty three sera and urine samples were collected from patients with acute schisto­somi­asis in diyala province, east of iraq in 2006.   their diseases were confirmed by find­ing   s. haematobium ova in urine exam...

The significance of the relationship between the three components of cardiolipin complementfixation antigen, viz. cardiolipin, natural lecithin, and cholesterol, was first studied by Maltaner and Maltaner (1945) in experiments with syphilitic pooled rabbit or human sera. Suitable sensitivity with regard to the routine sero-diagnosis of syphilis was obtained with the antigen formula: Cardiolipin...