We developed a droplet-based microfluidic screening system with an on-chip sampling probe integrating multi-channels for sample introduction, reagent merging and nanolitre-scale droplet generation, and a slotted-vial array sample presenting system. The present system was applied in protein crystallization conditions screening with an ultra-high sampling throughput up to 6000 h(-1) for different...

This paper uses numerical methods to characterize the crosstalk of small fluorescent molecules and molecular probes among aqueous droplets immersed in a continuous phase of hydrocarbons or fluorocarbons in microfluidic systems. Droplet-based biochemical assays rely on the reagents to remain isolated in individual droplets. It has been observed, however, that small and hydrophobic fluorescent mo...

The vast majority of droplet-based microfluidic devices are made from polydimethylsiloxane (PDMS). Unfortunately PDMS is not suitable for high frequency droplet generation at high operating pressure due to its low shear modulus. In this paper, we report the fabrication and testing of microfluidic devices using thermoset polyester (TPE). The optical characteristics of the fabricated devices were...

The generation of relativistic attosecond electron bunches is observed in three-dimensional, relativistic particle-in-cell simulations of the interaction of intense laser light with droplets. The electron bunches are emitted under certain angles which depend on the ratios of droplet radius to wavelength and plasma frequency to laser frequency. The mechanism behind the multi-MeV attosecond elect...

We present an experimental study of a new regime of monodisperse micro–droplet generation that we named the balloon regime. A dispersion of oil in water in a T–junction microfluidic system was studied. Several microfluidic devices having different cross– sections of the continuous and the dispersed phases micro–channels were tested. This new regime appears only for low dispersed phase velocity....

The miniaturization of droplet manipulation methods has led to drops being proposed as microreactors in many applications of biology and chemistry. In parallel, microfluidic methods have been applied to generate monodisperse emulsions for applications in the pharmaceuticals, cosmetics, and food industries. To date, microfluidic droplet production has been dominated by a few designs that use hyd...

This paper introduces to the fast system simulation approach for droplet-based microfluidics and its validation. Full custom centric design means the integration and functional combination of microfluidic operation units, such as sample droplet generation, dosing or mixing to a complete Lab-on-a-Chip system. Comparable to large scale integration in EDA a fast simulation for droplet-based microf...