Tumor cell proliferation requires both growth signals and sufficient cellular bioenergetics. The AMPactivatedprotein kinase (AMPK) pathway seemsdominant over the oncogenic signaling pathway suppressing cell proliferation. This study investigated the preclinical efficacy of targeting the tumor bioenergetic pathway using a glycolysis inhibitor 2-deoxyglucose (2DG) and AMPK agonists, AICAR andmetf...

DRP-1 and ZIPk are two members of the Death Associated Protein Ser/Thr Kinase (DAP-kinase) family, which function in different settings of cell death including autophagy. DAP kinases are very similar in their catalytic domains but differ substantially in their extra-catalytic domains. This difference is crucial for the significantly different modes of regulation and function among DAP kinases. ...

Caloric restriction (CR) is known to effectively elongate mammalian life-spans. The compound 2-deoxy-D-glucose (2DG), which is often used as an inhibitor of glucose utilization, is a mimetic agent of CR. In this study, we examined the changes of telomerase and Werner's syndrome RecQ (WRN) helicase after treatment with 2DG, because of the involvement of recQ helicase in the regulation of telomer...

The dorsal root potential (DRP) was measured on the lumbar dorsal roots of urethan anesthetized rats and evoked by stimulation of five separate inputs. In some experiments, the dorsal cord potential was recorded simultaneously. Stimulation of the L3 dorsal root produced a DRP on the L2 dorsal root containing the six components observed in the cat including the prolonged negative wave (DRP V of ...

PURPOSE The ketogenic diet (KD) has been used as an effective antiepileptic treatment for nearly a century. Inhibition of glycolysis and increased levels of ketone bodies are both known to contribute to the antiepileptic effects of the KD. Neuron-restrictive silencer factor (NRSF), also known as RE-1 silencing transcription factor (REST), is implicated in the antiepileptic effects of the glycol...

Salter-Venzon D, Watts AG. The role of hypothalamic ingestive behavior controllers in generating dehydration anorexia: a Fos mapping study. Am J Physiol Regul Integr Comp Physiol 295: R1009–R1019, 2008. First published July 30, 2008; doi:10.1152/ajpregu.90425.2008.— Giving rats 2.5% saline to drink for 3–5 days simply and reliably generates anorexia. Despite having the neurochemical and hormona...

In the present study, we propose Hyaluronic acid - 2-Deoxy-D-Glucose (HA-2DG) conjugate as a novel drug for treatment of COVID-19. silico molecular docking studies HA-2DG and (2DG) against four different SARS-CoV-2 viral protein (Mpro, RdRp, PLpro S protein) revealed that showed better binding affinity (-6.2, -7.2, -7.0 6.4 Kcal/mol) with all screened SASR-CoV-2 targets than antimetabolite 2DG ...

Targeting cancer cell metabolism is a new promising strategy to fight cancer. Metformin, a widely used antidiabetic agent, exerts antitumoral and antiproliferative action. In this study, the addition of metformin to 2-deoxyglucose (2DG) inhibited mitochondrial respiration and glycolysis in prostate cancer cells leading to a severe depletion in ATP. The combination of the two drugs was much more...

Six sites of 2-deoxyglucose (2DG)-induced lysis on three yeasts (Schizosaccharomyces pombe, Pichia farinosa, and Saccharomyces cerevisiae) coincided with the regions of growth of their glucan layers. Identification of the glucan layer as the site of lysis suggests a mechanism of attack by 2DG or by its derivatives. It is proposed that the glucan layer grows by addition of glucose into internal ...

Brief calorie restriction (CR; 20 days of 60% of ad libitum [AL] intake) improves insulin-stimulated glucose transport, concomitant with enhanced phosphorylation of Akt2. The purpose of this study was to determine whether Akt2 is essential for the calorie restriction-induced enhancement in skeletal muscle insulin sensitivity. We measured insulin-stimulated 2-deoxyglucose (2DG) uptake in isolate...