Poultry is an important source of protein and income for several households in Nigeria. However, the poultry industry threatened by outbreaks infectious diseases which may spread through interaction wild or migratory birds with poultry. The study aimed to detect morphologically identify Eimeria species from faecal samples chickens, pigeons African fire finches collected different farms Kaduna m...

Coccidiosis in chickens is caused by 7 species of Eimeria. Even though coccidiosis is a complex disease that can be caused by any combination of these species, most of the molecular research concerning chicken coccidiosis has been limited to Eimeria tenella. The present study describes the first large-scale analysis of expressed sequence tags (ESTs) generated primarily from second-stage merozoi...

An improved polymerase chain reaction (PCR)-based method for determining the species composition of Eimeria in poultry litter was developed by incorporating species-specific internal standards in the assay. Internal standard molecules were prepared by fusing seven different Eimeria species-specific intervening transcribed sequence 1 (ITS1) rDNA primer pairs to a non-Eimeria DNA molecule and by ...

in order to determine the involvement of nitric oxide in the pathogenesis of coccidiosis induced by eimeria, 30 chickens were challenged with mixed sporulated oocysts of four species of eimeria (e. acervulina, e. maxima, e. necatrix, and e. tenella) at 26 days of age. there was an increasing of oocyst shedding in the infected birds at 6, 10 and 14 days post-infection. histopathological examinat...

The relationship between oocyst dose and lesion score was evaluated in trials involving five field isolates each of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Each trial included an uninfected, unmedicated treatment, and at least three treatments of unmedicated birds given different doses of oocysts from a single isolate. In four trials each with E. acervulina and E. tenella, and ...

A cloned Eimeria acervulina gene (3-1E) was used to vaccinate chickens in ovo against coccidiosis, both alone and in combination with genes encoding interleukin (IL)-1, IL-2, IL-6, IL-8, IL-15, IL-16, IL-17, IL-18, or interferon (IFN)-gamma. Vaccination efficacy was assessed by increased serum anti-3-1E antibody titers, reduced fecal oocyst shedding, and enhanced body weight gain following expe...

OBJECTIVE We evaluated the epidemiology of antimicrobial resistance in enterococci from animal farms and the potential relation of resistance to antimicrobial use. METHODS Enterococci from faecal samples from 18 beef cattle, 18 dairy cattle, 18 swine, 13 chicken, and eight turkey farms were prospectively evaluated over a 6 year period from 1998 to 2003. RESULTS We evaluated 1256 isolates of...

The effect of feeding a Lactobacillus-based probiotic on intestinal intraepithelial lymphocyte (IEL) subpopulations and subsequent protection against coccidiosis was investigated in broiler chickens. Day-old male broilers were fed standard rations without control (CONT) or with a commercial probiotic (PROB) Primalac. Differences in IEL subpopulations were assessed by flow cytometry at 21 d post...

Three experiments were conducted with 9-d-old crossbred chicks to determine the effect of supplemental L-cysteine.HCl.H2O on tolerance to excess dietary Cd. Cd levels of 30 or 60 mg/kg added to a fully fortified corn-soybean meal diet depressed both body weight gain and gain:feed ratio and increased kidney Cd concentration. Supplemental cysteine (i.e., .59% L-cysteine.HCl.H2O) did not alleviate...

Cystic echinococcosis is a major emerging zoonosis in many Eastern European and Asian countries. Post slaughter examinations of 684 pig livers in Lithuania revealed significantly higher numbers of Echinococcus granulosus infections in animals from family farms (13.2%; 95% CI 10.7-16.2) as compared with those from industrial farms (4.1%; 95% CI 0.8-11.5). The prevalence was also significantly hi...