Endothelial 1 (E1) is identified as an endothelial cell secreted factor that stimulates collagen gel contraction by fibroblasts. This identification is based on (a) co-localization of stimulatory activity in endothelial cell conditioned media with synthetic E1 in reversed phase analysis; (b) removal of the activity from conditioned media with antiserum directed against E1; and (c) the activity ...

Cyclin E1 regulates the initiation of S-phase in cellular division. However, in many cancers, cyclin E1 is aberrantly overexpressed and this molecular phenotype correlates with increased tumor aggressiveness and poor patient survival. The molecular cause(s) of cyclin E1 abnormalities in cancers is poorly understood. Here, we show that cyclin E1 overexpression in cancer is promoted by dysregulat...

The E1 helicase from BPV and HPV16 interacts with Ubc9 to facilitate viral genome replication. We report that HPV11 E1 also interacts with Ubc9 in vitro and in the yeast two-hybrid system. Residues in E1 involved in oligomerization (353-435) were sufficient for binding to Ubc9 in vitro, but the origin-binding and ATPase domains were additionally required in yeast. Nuclear accumulation of BPV E1...

adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. in spite of their usefulness, the construction of adenoviral vectors (adv) is a cumbersome and lengthy process that is not readily amenable to the generat...

Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure o...

We prove a general result on the factorization of matrix-valued analytic functions. We deduce that if (E0, E1) and (F0, F1) are interpolation pairs with dense intersections, then under some conditions on the spaces E0, E1, F0 and F1, we have [E0⊗̂F0, E1⊗̂F1]θ = [E0, E1]θ⊗̂[F0, F1]θ, 0 < θ < 1. We find also conditions on the spaces E0, E1, F0 and F1 , so that the following holds [E0 ∨ ⊗F0, E1 ∨ ⊗F1...

The behavior of natural estrogens was examined by separately spiking 17β-estradiol (E2) and estrone (E1) into semicontinuous activated sludge reactors operated under aerobic, anaerobic and sequential anaerobic/aerobic conditions. Under aerobic and anaerobic conditions, E1 was formed from E2. The conversion of E1 to E2 was maximum conversion of E2 to E1 was higher than t E1 to E2. Overall, the d...

Two distinct types of cDNA clones encoding for the pyruvate dehydrogenase (PDH) E1 beta subunit were isolated from a human liver lambda gt11 cDNA library and characterized. These cDNA clones have identical nucleotide sequences for PDH E1 beta protein coding region but differ in their lengths and in the sequences of their 3'-untranslated regions. The smaller cDNA had an unusual polyadenylation s...

Background & Aims: Release of proinflammatory cytokines after traumatic brain injury (TBI) is a major cause of brain edema. Previous studies demonstrated that sex steroids decrease brain edema induced by TBI. In this study changes of brain cytokines after the administration of estrogen and progesterone 24 hours after TBI were evaluated. Materials and Methods: Female rats were divided into 7 gro...

KCNQ1 and KCNE1 (Q1 and E1) associate to form the slow delayed rectifier I(Ks) channels in the heart. A short stretch of eight amino acids at the extracellular end of S1 in Q1 (positions 140-147) harbors six arrhythmia-associated mutations. Some of these mutations affect the Q1 channel function only when coexpressed with E1, suggesting that this Q1 region may engage in the interaction with E1 c...