The human erythrocyte anion-exchanger isoform 1 (AE1) is a dimeric membrane protein that exchanges chloride for bicarbonate across the erythrocyte plasma membrane. Crystallographic studies suggest that the transmembrane anion channel lies at the interface between the two monomers, whereas kinetic analysis provides evidence that each monomer contains an anion channel. We have studied the structu...

Background. Platelets release two powerful vasoconstrictors thromboxane A2 (TXA2) and 5-hydroxytryptamine (5-HT). Animal studies have suggested that these two substances may act in a synergistic fashion to stimulate platelet activity and smooth muscle vasoconstriction. Methods and Results. To assess the interaction between TXA2 and 5-HT at the individual 5--HT receptor subtypes reported to medi...

Clones that confer the ability to produce N-acyl amino acids onto model cultured bacterial hosts are frequently identified in activity-based screens of soil DNA libraries. The N-acyl amino acids encoded by these clones are synthesized from acyl-(acylcarrier-protein)s (acyl-ACPs) and amino acids by a diverse group of bacterial enzymes referred to as N-acyl amino acid synthases (NASs). 2] The acy...

(EC 9o) mycelial inhibition (Y) were ABSTRACT examined by analysis of variance. Northover, J., and Matteoni, J. A. 1986. Resistance of Botrytis cinerea to benomyl and iprodione in Differences between means were evaluated vineyards and greenhouses after exposure to the fungicides alone or mixed with captan. Plant using the Student-Newman-Keuls multiple Disease 70:398-402. range test. A discrimin...

todays, essential oils from aromatic plants have been qualified as natural antioxidants and proposed as potential substitutes of synthetic antioxidants in food products. in this research chemical compositions and antioxidant activity of artimisia essential oil on oxidative stability of frying oil were analyzed. essential oil was extracted by hydro distillation and its chemical composition was d...

The extracellular homophilic-binding domain of the cadherins consists of 5 cadherin repeats (EC1-EC5). Studies on cadherin specificity have implicated the NH(2)-terminal EC1 domain in the homophilic binding interaction, but the roles of the other extracellular cadherin (EC) domains have not been evaluated. We have undertaken a systematic analysis of the binding properties of the entire cadherin...

We find a strong synergism between tumor necrosis factor (TNF) and bacteria or their products. Endotoxin-"free" recombinant TNF, even at very high doses (160 ,ug), did not alone cause hemorrhagic necrosis (RN) in the skin of normal mice. Similarly, TNF alone had a low systemic toxicity in tumorand pathogen-free mice. However, TNF given intravenously with nanogram quantities of the endotoxin lip...

FLOW-AID has the objective to develop and test an irrigation management system that can be used under deficit, when water availability and quality are limited. It intends to use innovative, but simple and affordable concepts usable for a variety of irrigation set-ups and constraints. Amongst others, it focuses on development of low-power wireless sensor networks for soil water monitoring. Six s...

GABAA receptors composed of 1, 2, 1 subunits are expressed in only a few areas of the brain and thus represent interesting drug targets. The pharmacological properties of this receptor subtype, however, are largely unknown. In the present study, we expressed 1 2 1-GABAA receptors in Xenopus laevis oocytes and analyzed their modulation by 21 ligands from 12 structural classes making use of the t...

Peroxisome proliferator-activated receptors (PPARs) are involved in the regulation of lipid and glucose metabolism. PPAR agonists improve insulin sensitivity and hyperglycemia and are effective in treating type 2 diabetes mellitus (T2DM), whereas PPAR agonists are used to treat dyslipidemia and atherosclerosis. The goal here was to examine the efficacy of a selective PPAR agonist {(S)-3-[3-(1-c...