نتایج جستجو برای: ecori and taqi from 14 combinations primer
تعداد نتایج: 17642383 فیلتر نتایج به سال:
The action of a number of restriction enzymes on DNA.RNA hybrids has been examined using hybrids synthesised with RNAs of cucumber mosaic virus as templates. The enzymes EcoRI, HindII, SalI, MspI, HhaI, AluI, TaqI and HaeIII cleaved the DNA strand of the hybrids (and possible also the RNA strand) into specific fragments. For four of these enzymes, HhaI, AluI, TaqI and HaeIII, comparison of the ...
Introduction & Objective: So far, despite the wide range of methods such as analytic methods used for differentiation of Mycoplasma, the diagnosis of Mycoplasma species is still difficult. Generally the low-level discriminatory power of serological methods because of the rapid changes in size and phase of the dominant antigens in the immune cell surface of Mycoplasmas greatly limits their app...
Introduction & Objective: So far, despite the wide range of methods such as analytic methods used for differentiation of Mycoplasma, the diagnosis of Mycoplasma species is still difficult. Generally the low-level discriminatory power of serological methods because of the rapid changes in size and phase of the dominant antigens in the immune cell surface of Mycoplasmas greatly limits their app...
Polymorphism. PstI restriction fragment length polymorphism (RFLP) in the ovine Aconitase 2 (AC02) gene. Source and Description of Probe. The probe is a .9kb AC02 cDNA from a porcine myocardial hgt 10 library (Zheng et al., 19901, cloned into the EcoRI site of pUC118. The insert was gel-purified and radiolabeled with [ c Y ~ ~ P I ~ C T P by the random primer method to specific activity > lo9 d...
abstract seedless barberry (berberis vulgaris l. var. asperma) is one of the few crops that is cultured only in iran and southern parts of khorasan provinces. the origin of this variety is unknown and there has not been any study aiming to identify phylogenic relationships of this plant with other species existing in iran. in this study, aflp markers based on four primer combinations (ecori/tru...
abstract as hybrid spawn is propagated asexually in edible mushrooms, producers may propagate it without the permission of the owner. for the protection of the owner right, there is a great need for a molecular certificate for each commercial line. the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to prepare molecular certifications and fin...
Studying genetic diversity is important because a decrease in genetic variability might result in a reduction of the plasticity of the crops to respond to changes in climate, pathogen populations, or agricultural practices. In this study, 72 Sardari wheat (Triticum aestivum L.) ecotypes were analyzed by AFLP markers and 17 phenotypic characters. Three pairs of EcoRI/MseI primer combinations pro...
The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8x8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic an...
Studying genetic diversity is important because a decrease in genetic variability might result in a reduction of the plasticity of the crops to respond to changes in climate, pathogen populations, or agricultural practices. In this study, 72 Sardari wheat (Triticum aestivum L.) ecotypes were analyzed by AFLP markers and 17 phenotypic characters. Three pairs of EcoRI/MseI primer combinations pro...
The study of phylogenetic relationships between 14 Colchicum taxa spread throughout Turkey was performed using a fluorescent-based amplified fragment length polymorphism (AFLP) technique. Five primer pair combinations were used in AFLP reactions. The data set was analyzed statistically using the NTSYS 2.1 software, and the neighbor-joining and maximum parsimony methods were implemented to gener...
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