The mechanism of inhibition of the veratryl alcohol oxidase activity of lignin peroxidase H2 (LiPH2) by EDTA was investigated. It was found that EDTA was decarboxylated and that cytochrome c, nitro blue tetrazolium, ferric iron, and molecular oxygen were reduced in a reaction mixture containing LiPH2, H2O2, veratryl alcohol, and EDTA. The reductive activity observed with LiPH2 followed first or...

Ethylenediaminetetraacetic acid (EDTA) is widely used in the life sciences as chelating ligand of metal ions. However, formation of supramolecular EDTA aggregates at pH > 8 has been reported, which may lead to artifactual assay results. When applied as a buffer component at pH ≈ 10 in differential scanning fluorimetry (TSA) using SYPRO Orange as fluorescent dye, we observed a sharp change in fl...

Ethylenediaminetetraacetic acid (EDTA) was developed by Franz Munz inGermanyduring the 1930s as an alternative to citric acid. About the same time, Frederick Bersworth synthesized EDTA in the United States using a different technique. He obtained aUSpatent for this technique in1945. EDTAwas approvedby the US Food and Drug Administration (FDA) as a food additive in 1947. Since the early 1950s, E...

Brucellosis is a zoonotic bacterial disease with worldwide distribution. A cross-sectional study was conducted to determine the serological prevalence of brucellosis in horses within Kano Metropolis. total 328 serum samples were collected for study. Modified Rose Bengal Plate Test (mRBPT) on all while Serum Agglutination EDTA (SAT-EDTA) mRBPT-positive samples. The metropolis found be 24.09 % (7...

Water-soluble polymers are attractive materials for pollutants removal thanks to their ability easily interact with soluble metal cations. In the present contribution, chemical modification of biocompatible and non-toxic poly(vinyl alcohol) (PVA) was achieved ethylene diamine tetraacetic acid (EDTA) groups, thus leading new water-soluble polymers, named PVA(EDTA). Modification carried out using...

Amidoximated algal/polyethyleneimine beads are successfully applied for the sorption of two rare earths elements (REEs, Ln(III)): La (light) and Dy (heavy). The properties characterized by FTIR SEM-EDX analyses. pH effect on metal is investigated with a double objective: optimizing recovery separation. In binary solutions, Dy(III) shows slightly higher affinity sorbent than La(III); without sig...

In ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia (PTCP), automated platelet counts are lower than actual counts because of EDTA-induced aggregation. Factors contributing to the incidence of EDTA-PTCP are unknown, and no study has assessed the prognosis of EDTA-PTCP patients. This retrospective study assessed characteristics in EDTA-PTCP patients and matched controls to...

EDTA inhibits horseradish peroxidase (HRP)-catalysed iodide oxidation in a concentration and pH-dependent manner. It is more effective at pH 6 than at lower pH values. A plot of log Kiapp. values as a function of pH yields a sigmoidal curve from which a pKa value of 5.4 can be calculated for an ionizable group on the catalytically active HRP for EDTA inhibition. Among the structural analogues o...