BACKGROUND The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material is necessary. METHODS Pairs of bilateral bone marrow biopsies sampled from 53 patients were de...

Soil sample from a site heavily contaminated with >10 wt.% zinc is sampled and extracted with aqueous solutions of ethylene diamine tetra-acetic cid (EDTA) that is a reagent frequently used to extract heavy metals in soil remediation. Three liquid/soil ratios (5/1, 20/1, and 100/1) were used n the extracting experiment. The molecular environment of the residual Zn in the EDTA-extract residues o...

A procedure involving alkaline extraction and solution 31P nuclear magnetic resonance (NMR) spectroscopy was developed and optimized for the characterization of P in animal manures (broiler, swine, beef cattle). Inclusion of ethylenediaminetetraacetic acid (EDTA) in the alkaline extraction solution recovered between 82 and 97% of the total P from the three manures, which represented a significa...

the alginate was extracted with six different methods from iran south seacoast algae, sargassum sp, and the percentage yield of alginate was determined. we divided our methods to two groups including acidic extraction and non-acidic. in acidic methods, hcl and h 2 so 4 were used as a detergent in extraction process and cacl 2 was exerted in non-acidic treatments. all treatments compared with ea...

Bio-oxidation of arsenic-bearing gold concentrate is generally slow and prone to passivation. To alleviate this concern, it pivotal explore the possible catalysts reduce passivation during bio-oxidation. In present work, enhancing effects Ag+, ethylenediamine tetraacetic acid (EDTA) oxalate (Ox) on bio-oxidation mechanisms were investigated by leaching experiments, thermodynamic calculation, XR...

Festuca ovina L. is a hyperaccumulating plant which has aroused considerableinterest with respect to its possible use for phytoremediation of contaminated soils. Thisstudy has been conducted to evaluate the potentials of F. ovina L. to serve as aphytoremediation plant in the cleaning up of Cu in the polluted soils and to identifyextraction efficiency of Ethylene Diamine Tetraacetic Acid (EDTA) ...

phytoextraction is a remediation technology for contaminated soils with lead (pb). the application of chelating agents can be resulted in high efficiency in this method. in current study, the effect of synthetic and natural chelates applicationon efficiency of lead phytoextraction from soil by carrot was investigated. the experiment factors were 1) six levels of pb (0, 100, 200, 300, 500 and 80...

Considerable interest has been focused on the role of myosin light chain LC(2) in the contraction of vertebrate striated muscle. A study was undertaken to further our investigations (Moss, R.L., G.G. Giulian, and M.L. Greaser, 1981, J. Biol. Chem., 257:8588-8591) of the effects of LC(2) removal upon contraction in skinned fibers from rabbit psoas muscles. Isometric tension and maximum velocity ...

In molecular studies, extraction can be time consuming and costly (especially in large scale studies), therefore some studies examine the success rate of amplification using alternative DNA isolation methods (without extraction). This study aims to determine most effective method Javan Plover sexing by comparing several methods, such as kit, alkaline lysis direct PCR.This used filter paper (5) ...