The present investigation was designed to study the spermicidal activity of lidocaine, a membrane stabilizer, and its combination with 2',4'-dichlorobenzamil hydrochloride, a Na+-Ca2+ exchange inhibitor, on human semen and spermatozoa separated from semen. Both drugs per se produced dose- and time-dependent reduction in motility of ejaculated human sperm. Lidocaine was found to potentiate the s...

INTRODUCTION Severely compromised spermatogenesis typical of men with virtual azoospermia or non-obstructive azoospermia requires an extreme search for spermatozoa. Our goal was to evaluate the usefulness of a meticulous search carried out in ejaculated or surgically retrieved specimens in achieving pre- and post-implantation embryo development. PATIENTS AND METHODS In a retrospective cohort ...

Freezing of boar spermatozoa includes the cryoprotectant glycerol, but renders low cryosurvival, owing to major changes in osmolarity during freezing/thawing. We hypothesize that aquaporins (AQPs) 7 and 9 adapt their membrane domain location to these osmotic challenges, thus maintaining sperm homeostasis. Western blotting (WB) and immunocytochemistry (ICC) at light and electron microscope level...

For many biological resources, larger males are more likely to be harvested than smaller ones. In such harvested populations, the remaining males are relatively small and participate in more matings than in pristine populations. Because the number of sperm passed to females generally decreases with decreasing male body size and increasing male mating frequency, the reproductive success of femal...

BACKGROUND To evaluate the safety of ICSI with epididymal sperm, this study compared children born after ICSI treatment with epididymal sperm and children conceived after IVF and ICSI with ejaculated sperm. Additionally, the results of a multidisciplinary, multicentre follow-up of the children conceived with epididymal sperm at 2 years of age are described. METHODS This follow-up study includ...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read with interest the commentary by Drs. Jan Tesarik and Maribel Galán-Lázaro (1) regarding the recently published practice recommendations for sperm DNA fragmentation (SDF) testing based on clinical scenarios by Agarwal et al. (2). Drs. Tesarik and Galán-Lázaro advocate the use of SDF testing in all cases of fert...

When ejaculated mouse spermatozoa were embedded in a plasminogen-containing insoluble protein substrate, a zone of proteolysis developed progressively, centered around the sperm head region. Lysis did not occur in absence of plasminogen or in presence of antibodies against the urokinase-type plasminogen activator (u-PA). Zymographic and immunological analyses confirmed the presence of u-PA in e...

The research aims to evaluate proportion of X-Y chromosom bearing sperm after sexing local ram semen with different combination BSA concentration. object was ten ejaculated ram, three years old. design used CRD (completely randomize design) four treatments concentration on upper and bottom layer (T1: 3% & 6%, T2: 4% T3: 5% 10%, T4: 6% 12%), 10 repetitions. Data analysis using variance Dunca...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. Ahmad, in his well-written commentary (1), shared his viewpoints about the utility of sperm DNA fragmentation (SDF) testing on each clinical scenario proposed in the practice recommendations by Agarwal et al. (2). He highlighted the pitfalls of relying on quantitative semen parameters in current practice and the n...

Seminolipid (sulphogalactosylalkylacylglycerol), the glycolipid that is specific for mammalian germ cells, is located exclusively in the outer leaflet of the sperm plasma membrane. In this study the lateral distribution of seminolipid on sperm heads has been investigated by indirect immunofluorescence labelling and detection with digital imaging fluorescence microscopy. In freshly ejaculated sp...