T he past several years have seen a significant resurgence of interest in cap i l l a ry electro ch ro m at ography(CEC), a hybrid technique that combines the high efficiency owing to the flow characteristics related to electroosmosis with the selectivity of high performance liquid chromatography(HPLC). In CEC, separations are generally carried out using fused silica capillaries packed with HPL...

Here we set forth the first from a series of reports devoted to history capillary electrophoresis. In this opening part, go more than two centuries back in time and revisit original discoveries electrolysis, electrophoresis electroosmosis. We emphasize essential role brilliant invention 1799 by Alessandro Volta, Voltaic pile, basically battery delivering constant-flow electricity, which has mad...

Here, the application of the gel-forming carbohydrate polymer, gellan gum, for the electrophoresis and isolation of DNA is detailed. Gellan gun forms gels in the presence of divalent metal cations, and the gels can be converted back to a solution by the addition of a chelating agent such as EDTA. Also, gellan electrophoresis gels can be formed using diamines. These gels are reversible by increa...

Label-free, nanoparticle-based plasmonic optical biosensing, combined with device miniaturization and microarray integration, has emerged as a promising approach for rapid, multiplexed biomolecular analysis. However, limited sensitivity prevents the wide use of such integrated label-free nanoplasmonic biosensors in clinical and life science applications where low-abundance biomolecule detection...

The field of AC electrokinetics is rapidly growing due to its ability to perform dynamic fluid and particle manipulation on the micro- and nano-scale, which is essential for Lab-on-a-Chip applications. AC electrokinetic phenomena use electric fields to generate forces that act on fluids or suspended particles (including those made of dielectric or biological material) and cause them to move in ...

Many biosensors are limited by long detection times that result from transport limitations of large macromolecules such as proteins which must reach the active surface in order to be detected. To overcome this difficulty, a common biosensor, the quartz crystal microbalance was modified with a microelectrode pattern capable of producing AC electroosmosis. The mixing pattern that is thereby gener...