Somatic embryos and plants were produced from mature and immature embryo derived callus of wheat. Immature embryo showed a better capacity for somatic embryogenesis and plant regeneration. Presence of 2, 4-D was shown to be essential for induction and maintenance of somatic embryogenesis. Organic nitrogenous additives (L-proline, L-asparagine and casein hydrolysate) marked an effect on somatic ...

St Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is an important warm season turf and pasture grass. In vitro tissue culture of St Augustinegrass could serve as an important mean for its improvement through genetic transformation as well as induced somaclonal variation. To optimize tissue culture conditions for plant regeneration of St Augustinegrass, tissue culture responses of 11 ex...

abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

The rapid direct and repetitive somatic embryogenesis in Coffea arabica and C. canephora genotypes was tested on Murashige and Skoog medium (MS) containing thidiazuron (TDZ) (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea) in concentrations of 2.27–11.35s M. Segments taken from cotyledon leaf, first leaf and stalk of regenerated plantlets produced clusters of somatic embryos directly from cut portions...

context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replicati...

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...

To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for cal...

An efficient in vitro protocol for plant production of North American ginseng has been established. The pretreatment of cotyledon explants with 1.0 M sucrose at 4◦C resulted in an improvement of embryo quality and, combined with a higher sucrose content (7%) in induction medium, improved the embryogenesis frequency from 40% to 75% and the number of embryos per explant from 10 to 21. The frequen...

somatic embryogenesis is a new approache to plant mass in vitro propagation. this method influences by elements and their components in medium. in order to investigate the effects of different media and it,s role on somatic embryogenesis of carrot, a cbd experiment with four replications (including 12 treatments) was conducted. to do this, carrot petioles (with 1 cm dimension) in induction phas...

background: polycystic ovaries syndrome (pcos) is the most common disorder in infertile women with ovulation sterility. there are some instances of abnormal endocrine in the women stricken by this syndrome and so the clinical results of ovulating induction in them, because of severe response of ovary against ovulating induction, are very discussing and may lead to different results from those w...