Abbreviations: EC – embryogenic calli; EFA – esterified fatty acids; FA – fatty acids; FAME – fatty acid methyl ester; FFA – free fatty acid; GC – gas chromatography; GC-MS – gas chromatography and mass spectrometry; HS – hypocotyl segments; HSC – hypocotyl segments with incipient calli formation; LCFA – long-chain fatty acid; MCFA – mediumchain fatty acids; NEC – non-embryogenic calli; PCA – p...

Tissue culture methods for plant regeneration have been developed for Lasiurus scindicus, Sorghum halepense and Urochloa panicoides using basal portion of young inflorescence as new and a potential source(s) of explants. Highly embryogenic cultures were initiated on Murashige and Skoog’s (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2-4,D). The regenera...

of embryogenic frequency 88.9 and 72.23 were recorded for stem explants. However, the greatest embryogenic calli fresh weights 14.68 and 9.65 (mg/jar) were derived from leaf explants. Moreover, growth of somatic embryos was investigated during 7weeks. The highest regeneration percentage was achieved for stem explants compared other explants and recorded 88.88 and 66.66 for directly or indirectl...

Embryogenic callus was induced from culturing immature embryos of three barley cvs. Dissa, Golden Promise and Igri. Maximum embryogenic callus formation was achieved by culturing immature embryos on CC and LS medium containing 2 mg/litre and 2.5 mg/litre 2,4dichlorophenoxyacetic acid (2,4-D) respectively. Embryogenic calli were frequently produced distinguished somatic embryos on these medium. ...

A large number of wheat recombinant lines were analysed for storage protein (glutenins) and active protein (betaamylase) polymorphism, using electrophoresis. The purpose of this paper was to outline the relationships between proteinogram types and bread making quality as well as between zymogram types and embryogenic potential. The experimental results emphasized that a great majority of the re...

Embryogenic cell suspensions of two elite Spanish grapevine cultivars (‘Albariño’ and ‘Tempranillo’) were successfully cryopreserved by encapsulation-dehydration. The method implied the encapsulation of cells in alginate beads, subsequent culture in liquid medium with 1 M sucrose for 4 days, and desiccation for 2-4 h in the flow of a laminar-flow bench, before immersing in liquid nitrogen. With...

Fresh or dry naked seed-explants of different maturity were cultured in vitro and their responses were investigated on a medium containing 2mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) as growth regulator. It was found that the explants at lower maturity had lower rates of callus induction and lower production of callus masses than those with the more matured ones in the original cultures. Howev...

Rice (Oryza sativa cv. 5272) embryogenic calli were obtained from mature zygotic embryos culture on Murashige & Skoog (1962) medium supplemented with 2.5 mg/l 2,4dichlorophenoxyacetic acid. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in ...

Endogenous indoleacetic acid (IAA) and cytokinin concentrations were measured by high performance liquid chromatography in leaf sections of an orchardgrass (Dactylis glomerata L.) genotype which exhibited a high capacity for somatic embryogenesis in vitro and in two genotypes that did not exhibit this capacity. The nonembryogenic genotypes contained 3- to 4-fold higher concentrations of zeatin,...