there are little information about growth properties of low pathogenic (lp) avian influenza virus (aiv) in embryonated chicken eggs (eces) at different incubation temperatures. knowledge of this information increases the quantity and quality of antigen in vaccine production process. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated (intra-allantoic) into 400, 11-day...

Newcastle Disease (ND) is a major viral disease in Indonesia. It is an RNA virus belongs to Paramyxovirinae. It is well known that RNA virus is easily to mutate. In some cases, this mutation could generate virulence alteration. It is noted that mutation of NDV which has avirulent amino acid sequence on the cleavage site, could mutate to be virulent Newcastle Disease Virus (NDV). It is needed to...

There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10(-5) dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into 400, 11-d...

Applicability of polymerase chain reaction (PCR) assay to detect Pasteurella multocida in experimentally infected embryonated chicken egg was assessed in the present study. PCR assay rapidly and specifically detected the genome of P. multocida in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. The sensitivity of detection was as low as 20 bacterial cells/ml...

During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in 310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conserve genes to search any BTV strains. Using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. Sensitivity of both molecular techniques evalua...

Flies were collected by sweep net from the vicinity of two small groups of "backyard" poultry (10-20 chickens per group) that had been identified as infected with exotic Newcastle disease virus (family Paramyxoviridae, genus avulavirus, ENDV) in Los Angeles County, CA, during the 2002-2003 END outbreak. Collected flies were subdivided into pools and homogenized in brain-heart infusion broth wit...

Influenza vaccines have long been manufactured in embryonated chicken eggs. This method has some problems such as a production period (about 6 months) and use of large amounts infectious pathogens. Recently, the recombinant subunit using baculovirus–insect cell system extensively investigated. In this system, viral immunodominant components can be produced more rapidly larger scale than convent...

Infectious Laryngotracheitis (ILT) is an acute highly contagious respiratory disease of chickens. It has significant economic importance due to mortalities and the decrease in egg production. In this study, twenty nine samples from different layer farms were collected outbreaks that occurred waset province, Iraq, at period November 2021 till March 2022 detect ILTV by molecular test through real...

Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fib...

objective: the objective of this study was to investigate the pathological alterations of methionine for chicken embryo.in recent years, amino acids are used in veterinary medicine for many purposes. they injected into hatching eggs to increase chick body weight and performance at hatch, but the adverse effects of amino acids have always been a major concern. there is scant information availabl...