نتایج جستجو برای: embryonic screening
تعداد نتایج: 307410 فیلتر نتایج به سال:
BioTechniques 349 Embryonic stem (ES) cells were established from the inner cell mass of blastocysts and have been shown to spontaneously differentiate into multiple cell types representative of all three embryonic germ layers, both in teratomas after grafting or in vitro under appropriate conditions (1,2). ES cells can be maintained in their pluripotent state if cultured in the presence of leu...
Two recent studies, including one in this issue of Cell Stem Cell, have identified novel regulators of embryonic stem cell (ESC) self-renewal using genome-wide RNAi screens in mouse ESCs (Ding et al., 2009; Hu et al., 2009) and have further expanded the repertoire of factors that regulate ESC identity.
Introduction: The article describes a new method of tissue engineering, which is based on the use three-dimensional multicellular constructs consisting stem cells that mimic native in vivo – organoids.
 3D cell cultures: currently existing model systems cultures are described.
 Characteristics organoids and strategies for their culturing: main approaches to fabrication using pluripote...
Members the DNA Methyltransferases (DNMT) gene family have been shown to play fundamental roles in regulating embryonic growth and development from embryonic fertilization to postnatal life; through regulating the establishment and/or maintenance of specific epigenetic marks. The present study was conducted to identify potential reported mutations within the exon 33 of DNMT-1, intron 4 of DNMT-...
1. Abstract 2. Introduction 3. Methods and Materials 3.1. Generation of the targeting vector 3.2. Electroporation, selection and screening of embryonic stem (ES) cells 3.3. Blastocyst injection and animal breeding 3.4. Isolation of RNA, Quantitative PCR and whole-mount in situ hybridization 3.5. Skeletal X-rays and staining 4. Results 4.1. Generation of Zic3 4.2. Upregulation of Zic3 expression...
Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate re...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید