The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin (HSA) was investigated using indirect chiral high performance liquid chromatography (HPLC) and ultrafiltration techniques. S-(-)-1-(1-naphthyl)-ethylamine (S-NEA) was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for...

The stereoselective toxicokinetics of ethofumesate enantiomers following a single intravenous (i.v.) administration at doses of 30 mg/kg were investigated in rabbits. Plasma concentrations of (+)- and (-)-ethofumesate were analyzed by a validated chiral HPLC method that involved extraction of plasma with organic solvent followed by separation on a cellulose-Tris-(3,5-dimethylphenylcarbamate)-ba...

This review summarizes recent developments in the field of enantiomer separation by capillary electrochromatography using fritless packed columns. Various enantiomers have been separated by employing fritless packed columns prepared in a fused silica capillary either by the immobilization of chiral packing materials by sintering or sol-gel technology or by in situ polymerization of a mixture co...

A novel, simple, precise, and stability-indicating stereoselective method was developed and validated for the accurate quantification of the enantiomer in the drug substance and pharmaceutical dosage forms of Rosuvastatin Calcium. The method is capable of quantifying the enantiomer in the presence of other related substances. The chromatographic separation was achieved with an immobilized cellu...

A reversed phase enantioselective high performance liquid chromatographic method was developed and validated for determination ofS-enantiomer in Cinacalcet drug substance. The enantiomers of Cinacalcet were resolved on a Chiralcel-OJH (250mmx4.6mm, 5microns) column using a mobile phase system containing n-Hexane, ethanol and n-butyl amine (90:10:1). The resolution between enantiomers was found ...

Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affinity CE) with some proteins and by cyclodextrin electrokinetic chromatography (CDEKC) with some charged cyclodextrins (CDs). Many successful enantiomer separations are demonstrated f...

Abstract: Background: The purification of drug enantiomers is an essential technique because pharmacological activity differs depending on individual enantiomers. Nowadays, supercritical fluid technology utilized for various purposes in the pharmaceutical field. Methodology: In this study, efficient and streamlined method development strategy finding effective analytical chromatography (SFC) pr...

Chemical warfare nerve agents (CWNAs) are extremely toxic organophosphorus compounds that contain a chiral phosphorus center. Undirected synthesis of G-type CWNAs produces stereoisomers of tabun, sarin, soman, and cyclosarin (GA, GB, GD, and GF, respectively). Analytical-scale methods were developed using a supercritical fluid chromatography (SFC) system in tandem with a mass spectrometer for t...

A simple reversed-phase chiral HPLC method has been developed and validated for direct separation of the enantiomers of zaltoprofen. Separation of the enantiomers was tested on numerous commercial chiral HPLC columns. Separation was best (resolution, RS = 3) on a Chiralcel OJ-RH stationary phase. Typical retention times of the S and R enantiomers were approximately 14 and 16 min, respectively. ...

Crystallization of a solution with high enantiomeric excess can generate a mixture of crystals of the desired enantiomer and the racemic compound. Using a mixture of S-/RS-ibuprofen crystals as a model, we demonstrated that magnetic levitation (MagLev) is a useful technique for analysis, separation and enantioenrichment of chiral/racemic products.