Enzymatic saccharification (Hydrolysis) is one of the technologies to hydrolyze lignocellulosic biomass. In this study, Bangladeshi bagasse is used as feedstock for enzymatic saccharification and ethanol fermentation. Cutter mill and Mechano-chemical treatment (Ball mill) are used for pretreatment. The main objective of this study is to investigate the effect of substrate concentration, pretrea...

proper conditions for producing crude beta-galactosidase from waste materials were de-termined. this enzyme is to be used in the production of lactose-hydrolyzed milk. whey permeate was used as a basic medium. twenty seven treatments were developed by 3 vary-ing factors of: yeast extract (1, 2, and 3 %), wheat steep liquor (1, 2, and 3 %), and whey powder (0.5, 1, and 1.5 %). crude enzyme extra...

Acid and enzymatic hydrolysis of cassava starch to glucose (fermentable sugar) were investigated and compared. And the effects of acid concentration, pH, temperature and time on the yield of glucose were studied. Experiments were carried out at a temperature range of (60 – 100)C between 30 minutes and 4 hours. (0.2 – 1.0)M strength of H2SO4 acid was used and pH values range of 4 – 7 was conside...

BACKGROUND The efficiency of enzymatic hydrolysis is reduced by the structural properties of cellulose. Although efforts have been made to explain the mechanism of enzymatic hydrolysis of cellulose by considering the interaction of cellulolytic enzymes with cellulose or the changes in the structure of cellulose during enzymatic hydrolysis, the process of cellulose hydrolysis is not yet fully un...

Conventional production of instant coffee is based on solubilisation of polysaccharides present in roasted coffee. Higher process temperatures increase the solubilisation yield, but also lead to carbohydrate degradation and formation of undesirable volatile compounds. Enzymatic hydrolysis of roasted coffee is an alternative to minimize carbohydrate degradation. In this work, products obtained f...

Enzymatic hydrolysis of pre-treated wheat straw has been studied. Several additives have checked as potential improvers glucose yield in the enzymatic hydrolysis, trying to avoid cellulases inhibition by lignin a cause decrease saccharification rate lignocellulosic biomass. Non-ionic surfactants, such Tween 80 (poly(oxyethylene)20-sorbitan-monooleate) and polyethylene glycol-6000, proteins, bov...

In the current work, a model for enzymatic hydrolysis and SSF has been reviewed and modified. Parameters referring to the enzymatic hydrolysis were estimated against new experimental data by using a non-linear least square regression tool in Matlab (MathWorks Inc.). To enable modeling of different substrates a new parameter accounting for different substrates susceptibility to enzymatic hydroly...

Slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. Maximum glucose yi...

Studies over the past 20 years on the metabolism of carbohydrates have implicated glucuronolactone as a precursor of several compounds of physiological interest; namely, L-xylulose (1, 2), ascorbic acid (3, 4), and furan2,5-dicarboxylic acid (5). Studies in viva from two laboratories, demonstrating that mammalian tissues can oxidize glucuronolactone to CO2 (6) but fail to oxidize glucuronic aci...