l glutaminase, a therapeutic enzyme obtained from marine actinomycetes gains importance because of their adaptability to varied environmental factors. an experimental study was carried out to isolate the l glutaminase producing actinomycetes from the marine sediment of thoothukudi. marine sediment sample was collected from six different locations of the thoothukudi coastal ecosystem, enriched w...

abstract digestive enzyme inhibitors are proteinacious or nonproteinacious compounds which reduce an enzyme activity through attaching to its active site and/or its substrate. nowadays, plant enzyme inhibitors are of great importance because 1) these have considerable effects on insect digestive enzymes and as a result on their development; and 2) the transgenic plants expressing them are safe....

polyphenol oxidase (ppo) from tomatoes was extracted and partially purified through  (nh4)2so4 precipitation, dialysis and ion exchange chromatography. the activity of polyphenol oxidase was investigated in solanum lycopersicum. spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (vmax) and michealis - menten constant (km). of the ...

Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37 ?C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46 was obtained in 2%w/v Sodium alginate solution. Investiga...

Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through  (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...

Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through  (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

the main objective of this study was to purify and characterize an esterase from shewanella sp f88. the enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and q-sepharore chromatography. molecular weight of the enzyme was 62.3 kda according to sds-page data. the enzyme showed an optimum activity at ph 6.5 and 58 ˚c. evo...