Background: Polygalacturonase is an important pectin degrading enzyme. The western honey bee (Apis mellifera L.) collects pollens from different flowers which are rich source of pectin. The microbiota in the gut of honey bee, release polygalacturonase enzyme and help in pectin digestion. This study aims to isolate and characterize novel polygalacturonase producing bacterial strain from honey be...

Polygalacturonases (PG) represent an important member of pectinases group of enzymes with immense industrial applications. A fungal strain Aspergillus niger MTCC478 was used for the production of polygalacturonase both under submerged and solid-state fermentation condition. Further its production was optimized under solid-state fermentation condition with media comprising of wheat bran and tea ...

conclusions bacteria from biological waste were competent producers of pg and must be used on an industrial scale to cope with the demand of pg in the food industry. results among the bacillus, bacillus vallismortis (jq990307) isolated from mango was the most competent producer of pg; producing up to 4.4 u/µl. amongst pseudomonas, pseudomonas aeruginosa (jq990314) isolated from oranges was the ...

The necrotrophic fungal pathogen Sclerotinia sclerotiorum secretes oxalic acid and endo-polygalacturonase (endo-PG) in host plants. Oxalic acid acidifies the plant tissue to values more suitable to endo-PG activity. However, we observed that the infected soybean seedlings possessed a pH of 3.8, which is below that optimal for endo-PG activity (4.5 to 5.0). We investigated, therefore, the effect...

Tomato fruit ripening is accompanied by extensive degradation of pectic cell wall components. This is thought to be due to the action of a single enzyme, polygalacturonase, whose activity is controlled, at least in part, at the level of gene expression. At the onset of tomato fruit ripening, polygalacturonase enzyme activity, mRNA levels, and relative rate of gene transcription all increase dra...

ascochyta rabiei is one of the devastaining fungi pathogens of chick-pea (cicer arietinum l.) crops. in the present investigation, forty-two iranian isolates of ascochyta rabiei fungi (including highly, weakly and moderate virulent isolates), whose pathogenisities were determined previously, were studied. polygalacturonase is a main factor in the initiation of ascochyta blight disease. therefor...

Abbreviations: ARP: acidic ribosomal protein CHS: chalcone synthase DDRT-PCR: differential display reverse transcriptase polymerase chain reaction DETs: differentially expressed transcripts DPA: days post anthesis ESTs: expressed sequence tags MBD: methyl CpG binding domain OPT: oligopeptide transporter protein PCR: polymerase chain reaction PG: polygalacturonase enzyme RRF: ribosome recycling ...

Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The maximal activity value of polygalacturonase (Pg) (30U.g) was obtained using wheat bran as carbon source while maximal pectin lyase (Pl) (2000 U.g)...